TSLPR (TSLP-R) Antikörper (APC)

Antigen: TSLPR (TSLP-R)

Klonalität: Monoklonal (1B4)

Wirt: Maus

Reaktivität: Human

Konjugat: APC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN179924

Produktbeschreibung

Immunogen: Human TSLP-R:Fc protein

Isotyp: IgG1, kappa chain

Klon: 1B4

Beschreibung: TSLP-R, also known as thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, and IL-XR, is a type I membrane receptor that forms a functional heterodimeric complex with IL-7R to bind TSLP. The TSLP-R contains a WSXWS motif required for proper protein folding and a box1 motif important for association with the JAKs. TSLP-R has a predicted molecular weight approximately 40 kD, and two isoforms have been reported that are produced by alternative splicing. The TSLP-R is expressed preferentially in myeloid cells including dendritic cells and activated monocytes, and is weakly expressed in T cells. Expression has also been reported in heart, skeletal muscle, and kidney tissues. TSLP binding to the heterodimeric functional receptor (TSLP-R and IL-7R) activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Ligand receptor interactions haves been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergic diseases. Distribution: Expressed preferentially in myeloid cells including dendritic cells and activated monocytes, weakly expressed in T cells. Also expressed in heart, skeletal muscle, and kidney Function: TSLP binding to functional receptor activates JAK2, STAT3 and STAT5 to stimulate cell proliferation. Has been implicated in the development of the hematopoietic system, dendritic cell maturation, and the maintenance and polarization of human Th2 memory T cells in allergic diseases. Synonyms: Thymic stromal lymphopoietin protein receptor, cytokine receptor-like 2, CRL2, CRLF2, IL-XR, TSLP-R Structure: Type I membrane receptor, forms functional heterodimeric complex with IL-7R to bind TSLP. Contains WSXWS motif required for proper protein folding and a box1 motif that is important for association with the JAKs. Predicted molecular weight approximately 40 kD, two isoforms produced by alternative splicing.

Spezifität: Together with IL-7R binds TSLP.

Anwendungen

Applikationshinweise: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (ABIN159342/ABIN159343) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG (ABIN159908) second step, followed by SAv-PE (ABIN159899)). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar