Integrin B7 Antikörper

Antigen: Integrin B7

Klonalität: Monoklonal (FIB504)

Wirt: Ratte

Reaktivität: Maus, Human

Applikation: Durchflusszytometrie (FACS), Blockierende Antikörper (Inhibition)

Produktnummer: ABIN179921

Produktbeschreibung

Immunogen: TK1 cells

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: FIB504

Beschreibung: Integrin beta7 is a 130 kD glycoprotein, also known as integin betap. It is a member of the Ig superfamily. In association with integrin alpha4 or alphaE chain, beta7 forms alpha4/beta7 or alphaE/beta7 heterodimer. alpha4/beta7 (CD49d/beta7, LPAM-1) is expressed on majority of peripheral lymphocytes, small subsets of thymocytes and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion, some hematopoietic progenitor cells migration. alphaE/beta7 (CD103/beta7,alphaIEL/beta7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103/beta7 complex is thought to play a role in lymphocyte retention via interaction with its lignd E-Cadherin. The Fib504 antibody has been reported to react with mouse and human beta7 integrin and to block beta7 integrin-mediated cell adhesion in in vitro and in vivo studies. Distribution: CD49d/ß7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors, CD103/ß7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes Function: Lymphocyte adhesion, hematopoietic progenitor cells migration Synonyms: beta7 Integrin, integrin betap Structure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: blocking of cell adhesion. The FIB504 antibody cross-reacts with mouse Integrin beta7. The LEAF(TM) purified FIB27 antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Integrin B7 antibody