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Antikörper
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anti-CD34 (CD34) Antikörper (Biotin)
CD34 (CD34) Antikörper (Biotin)
| Antigen |
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| Klonalität |
Monoklonal (4H11) |
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Wirt
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Reaktivität
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Human (214), Maus (129), Ratte (Rattus) (34), Hund (28), Rind (Kuh) (15), Schwein (15), Kaninchen (15), Affe (7), Primate (5), Primaten (3), Rhesusaffen (2)
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Konjugat
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FITC (43), PE (30), Biotin (24), APC (10), Alexa Fluor 647 (8), RPE (6), PE,Cy5 (4), PerCP,Cy5.5 (4), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 700 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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Applikation
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Durchflusszytometrie (FACS) (249), Western Blot (WB) (108), Immunhistochemie (Paraffinschnitte) (IHC (p)) (70), Immunfluoreszenz (IF) (56), Immunpräzipitation (IP) (50), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (47), ELISA (29), Immunhistochemie (IHC) (16), Immunzytochemie (ICC) (12), Enzyme Immunoassay (EIA) (8), Funktionale Studien (Func) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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| Produktnummer |
ABIN179735 |
| Menge |
100 µg |
| Preis |
Produkt für diese Region nicht verfügbar.
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| Lieferung nach |
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Weitere Bezeichnung
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CD34
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Immunogen
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human peripheral leucocytes
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Isotyp
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IgG1, kappa chain
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Klon
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4H11
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Beschreibung
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CD34 is a type I monomeric sialomucin-like glycophophoprotein with the molecular weight about 105-120 kD, also know as gp105-120. It is selectively expressed on majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymic cleavage, four groups of epitopes of CD34 have been described. 4H11 antibody recognized epitope is in class III group which is resistant to sialidase/glycolyprotease and chymopapain treatment. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands. Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts Function: Cell adhesion Synonyms: gp105-120, Mucosialin, My10 Structure: 105-120 kD single chain mucin-like glycoprotein
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Applikationshinweise
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
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Reinigung
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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Lagerung
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The antibody solution should be stored undiluted at 4°C. Do not freeze.
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Forschungsgebiet
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CD Antigene
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "CD34 (CD34)", Typ "Antikörper" finden
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Wirte
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Maus (170), Ratte (84), Kaninchen (60), Hamster (8), Huhn (4)
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Reaktivitäten
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Human (214), Maus (129), Ratte (Rattus) (34), Hund (28), Rind (Kuh) (15), Schwein (15), Kaninchen (15), Affe (7), Primate (5), Primaten (3), Rhesusaffen (2)
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Applikationen
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Durchflusszytometrie (FACS) (249), Western Blot (WB) (108), Immunhistochemie (Paraffinschnitte) (IHC (p)) (70), Immunfluoreszenz (IF) (56), Immunpräzipitation (IP) (50), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (47), ELISA (29), Immunhistochemie (IHC) (16), Immunzytochemie (ICC) (12), Enzyme Immunoassay (EIA) (8), Funktionale Studien (Func) (8), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), Dot Blot (Dot) (2), Immunoelectron Microscopy (IEM) (2)
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Konjugate
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FITC (43), PE (30), Biotin (24), APC (10), Alexa Fluor 647 (8), RPE (6), PE,Cy5 (4), PerCP,Cy5.5 (4), Alexa Fluor 488 (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 700 (2), Cy3 (2), Cy5 (2), Cy5,PE (2), Cy5.5 (2), Cy7 (2), Cy7,PE (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Cy5.5,PE (1), PE,DY647 (1), PE-Dyomics 647 (1), Pacific Blue (1)
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Epitope
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AA 32-290 (1), pTyr330 (1)
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Alternativen 
