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Antikörper
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anti-Phosphotyrosine (0) Antikörper (Alexa Fluor 647)
Phosphotyrosine (0) Antikörper (Alexa Fluor 647)
| Antigen |
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| Klonalität |
Monoklonal (PY20) |
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Wirt
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Reaktivität
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Konjugat
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Immunfluoreszenz (IF)
Western Blot (WB) (165), Immunpräzipitation (IP) (100), ELISA (62), Enzyme Immunoassay (EIA) (49), Immunfluoreszenz (IF) (45), Durchflusszytometrie (FACS) (24), Immunhistochemie (IHC) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (18), Immunzytochemie (ICC) (13), Immunhistochemie (Paraffinschnitte) (IHC (p)) (7), Radioimmunoassay (RIA) (2), To be determined by user (TBD) (2)
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| Produktnummer |
ABIN179733 |
| Menge |
100 µg |
| Preis |
Produkt für diese Region nicht verfügbar.
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| Lieferung nach |
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Weitere Bezeichnung
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Phosphotyrosine
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Immunogen
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KLH-conjugated phosphotyrosine
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Isotyp
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IgG2b, kappa chain
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Klon
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PY20
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Beschreibung
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Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining. Distribution: Phospho-Specific Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
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Applikationshinweise
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Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunofluorescence microscopy. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Reinigung
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The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
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Buffer
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
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Lagerung
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The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Forschungsgebiet
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Aminosäuren, Proteinmodifikationen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Glenney, Zokas, Kamps: "Monoclonal antibodies to phosphotyrosine." in: Journal of immunological methods, Vol. 109, Issue 2, pp. 277-85, 1988 (PubMed).
Vuori, Ruoslahti: "Tyrosine phosphorylation of p130Cas and cortactin accompanies integrin-mediated cell adhesion to extracellular matrix." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22259-62, 1995 (PubMed).
Prahalad, Calvo, Waechter et al.: "Regulation of MDCK cell-substratum adhesion by RhoA and myosin light chain kinase after ATP depletion." in: American journal of physiology. Cell physiology, Vol. 286, Issue 3, pp. C693-707, 2004 (PubMed).
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Alternativen zu Antigen "Phosphotyrosine (0)", Typ "Antikörper" finden
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Wirte
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Maus (167), Kaninchen (34), Ziege (3), Armenischer Hamster (2)
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Reaktivitäten
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Human (25), Ratte (Rattus) (19), Maus (14), Hund (13), Alle Spezies (8), Ziege (5), Diverse Spezies (4), Huhn (1), Rind (Kuh) (1)
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Applikationen
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Western Blot (WB) (165), Immunpräzipitation (IP) (100), ELISA (62), Enzyme Immunoassay (EIA) (49), Immunfluoreszenz (IF) (45), Durchflusszytometrie (FACS) (24), Immunhistochemie (IHC) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (18), Immunzytochemie (ICC) (13), Immunhistochemie (Paraffinschnitte) (IHC (p)) (7), Radioimmunoassay (RIA) (2), To be determined by user (TBD) (2)
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Konjugate
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Biotin (30), HRP (22), FITC (17), Alkaline Phosphatase (AP) (8), PE (8), Agarose Beads (5), RPE (2), TAMRA (2), Alexa Fluor 488 (1), Rhodamine (1)
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