You are viewing an incomplete version of our website. Please click to reload the website as full version.

Nestin Antikörper (NES) (FITC)

Details für Produkt anti-NES Antikörper Nr. ABIN1774766, Anbieter: Anmelden zum Anzeigen
Antigen
  • paranemin
  • NES
  • AA166324
  • C78523
  • ESTM46
  • nestin
  • NES
  • nes
  • Nes
Reaktivität
Human
249
74
67
17
4
4
3
3
2
2
2
2
1
1
1
Wirt
Maus
168
117
9
6
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Nestin Antikörper ist konjugiert mit FITC
12
11
11
9
6
6
5
5
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF)
205
108
102
95
89
89
80
47
15
13
11
5
4
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

'Independent Validation' Siegel
Chargennummer I030314
Validierte Anwendung Immunofluorescence
Positivkontrolle PC-3 cells
Negativkontrolle MCF-7 cells
Bewertung Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
Primärantikörper
Kontrollen
  • Positive control: PC3 Nestin positive prostate adenocarcinoma cells (specimen known to contain high
  • levels the target protein – 100% positive) from ATCC.
  • Negative Control: MCF7 Nestin negative breast adenocarcinoma cells (specimen known to contain very
  • low levels of the target protein - <13% positive) from ATCC.
Protokoll
  • Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
  • Fixed cells were rinsed three times in PBS for 5 min each at RT.
  • Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
  • Cells were rinsed two times in 1X PBS.
  • Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
  • Cells were rinsed three times in PBS for 5 min each at RT.
  • Coverslips were mounted on slides with DAPI-Fluoromount-G.
  • Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
Anmerkungen The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
Validierungsbilder
Immunofluorescence validation image for anti-Nestin (NES) antibody (FITC) (ABIN1774766) Legend: Immunofluorescence. Nestin (green fluorescence) is present in positive contro...
Immunogen A 150-amino-acid fragment from the cloned human nestin conjugated to GST
Klon [10C2] (also known as 2C1-3A11)
Isotyp IgG1
Spezifität This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
Reinigung Protein G purified
Andere Bezeichnung Nestin (NES Antibody Abstract)
Hintergrund Neural Stem Cell Marker
Gen-ID 10763
OMIM 600915
UniProt P48681
Forschungsgebiet Angiogenesis, Lineage Markers
Applikationshinweise Optimal antibody dilution should be determined by titration.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link ®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.