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Nestin Antikörper (FITC)

NES Reaktivität: Human IF, ICC Wirt: Maus Monoclonal 10C2 FITC
Produktnummer ABIN1774766
  • Target Alle Nestin (NES) Antikörper anzeigen
    Nestin (NES)
    Reaktivität
    • 131
    • 69
    • 51
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 103
    • 38
    • 4
    • 2
    • 1
    Maus
    Klonalität
    • 108
    • 40
    Monoklonal
    Konjugat
    • 66
    • 10
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    Dieser Nestin Antikörper ist konjugiert mit FITC
    Applikation
    • 82
    • 51
    • 49
    • 48
    • 40
    • 40
    • 39
    • 28
    • 25
    • 15
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
    Aufreinigung
    Protein G purified
    Immunogen
    A 150-amino-acid fragment from the cloned human nestin conjugated to GST
    Klon
    10C2
    Isotyp
    IgG1
    Top Product
    Discover our top product NES Primärantikörper
  • Applikationshinweise
    Optimal antibody dilution should be determined by titration.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029824 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Ensigna Biosystems
    No.
    #029824
    Datum
    06.11.2014
    Antigen
    Chargennummer
    I030314
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    PC-3 cells
    Negativkontrolle
    MCF-7 cells
    Bewertung
    Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Nestin (NES) (FITC)
    • Catalog number: ABIN1774766
    • Lot number: I030314
    • Dilution: 1:75, 1:100
    Sekundärantikörper
    Full Protocol
    • Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
    • Cells were rinsed two times in 1X PBS.
    • Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with DAPI-Fluoromount-G.
    • Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
    Anmerkungen
    The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target
    Nestin (NES)
    Andere Bezeichnung
    Nestin (NES Produkte)
    Synonyme
    paranemin antikoerper, NES antikoerper, AA166324 antikoerper, C78523 antikoerper, ESTM46 antikoerper, nestin antikoerper, nestin S homeolog antikoerper, NES antikoerper, nes.S antikoerper, Nes antikoerper, nes antikoerper
    Hintergrund
    Neural Stem Cell Marker
    Gen-ID
    407242
    OMIM
    600915
    UniProt
    P18175
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