GABRA3 Antikörper (AA 29-43)

Produktnummer ABIN1742459

Produktdetails anti-GABRA3 Antikörper

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Bindungsspezifität: AA 29-43

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Spezifität: Specific for GABA-A receptor alpha3.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide (aa 29-43 of rat GABA-A receptor alpha3 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500

Kommentare:

WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Referenzen für anti-GABRA3 Antikörper (ABIN1742459)

Tzanoulinou, García-Mompó, Castillo-Gómez, Veenit, Nacher, Sandi: "Long-term behavioral programming induced by peripuberty stress in rats is accompanied by GABAergic-related alterations in the Amygdala." in: PLoS ONE, Vol. 9, Issue 4, pp. e94666, (2014) (PubMed).

Details zu GABRA3

Target: GABRA3 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 3 (GABRA3))

Andere Bezeichnung: GABA-A Receptor Alpha3 (GABRA3 Produkte)

Synonyme: GABRA3 antikoerper, Gabra-3 antikoerper, si:ch211-113k9.1 antikoerper, gamma-aminobutyric acid type A receptor alpha3 subunit antikoerper, gamma-aminobutyric acid (GABA) A receptor, alpha 3 antikoerper, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 3 antikoerper, gamma-aminobutyric acid receptor subunit alpha-3 antikoerper, GABRA3 antikoerper, gabra3 antikoerper, Gabra3 antikoerper, LOC100653496 antikoerper

Hintergrund: Synonyms: GABA(A) R alpha3