gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) Antikörper

Details zu Produkt Nr. ABIN1742455, Anbieter: Anmelden zum Anzeigen
Antigen
  • ECA4
  • EJM
  • EJM5
  • zgc:153566
  • Gabra-1
  • gamma-aminobutyric acid type A receptor alpha1 subunit
  • gamma-aminobutyric acid (GABA) A receptor, alpha 1
  • gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1
  • gamma-aminobutyric acid receptor subunit alpha-1
  • GABRA1
  • Gabra1
  • gabra1
  • LOC100653509
Epitop
AA 28-43
38
35
27
18
18
6
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Ratte (Rattus), Maus
154
154
128
24
11
11
8
5
4
3
3
2
2
2
1
1
1
Wirt
Maus
96
82
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
158
77
77
73
25
18
16
16
11
3
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Klon 53D3
Isotyp IgM
Spezifität Specific for GABA-A receptor alpha1.
Reinigung purified IgM. Azide was added before lyophilization.
Andere Bezeichnung GABA-A Receptor Alpha1 (GABRA1 Antibody Abstract)
Hintergrund Synonyms: GABA(A) R alpha1
Applikationshinweise WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 500
Kommentare

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA). Best results are obtained by application of antibodies on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Lagerung -20 °C
Informationen zur Lagerung Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bilder des Herstellers
Western Blotting (WB) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) dilution: 1 : 1000, sample: unboiled synaptic membrane fraction of rat brain (LP1)
Immunocytochemistry (ICC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) Indirect immunostaining of hippocampus neurons with anti-GABA-A receptor α1 (dilution...
Immunohistochemistry (IHC) image for anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1) (AA 28-43) antibody (ABIN1742455) Indirect immunostaining of mouse cerebellum and main olfactory bulb vibratome section...
Produkt verwendet in: Acuna, Liu, Südhof: "How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs." in: Neuron, Vol. 91, Issue 4, pp. 792-807, 2016 (PubMed).

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