33 Kda of The Oxygen Evolving Complex (Oec) of Psii (Psbo) Antikörper

Antigen: 33 Kda of The Oxygen Evolving Complex (Oec) of Psii (Psbo)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Barley (Hordeum vulgare), Jack Pine (Pinus banksiana), Spinat (Spinacia oleracea), Chlamydomonas reinhardtii (C. reinhardtii), Synechococcus PCC 7942

Applikation: Western Blot (WB)

Produktnummer: ABIN173363

Produktbeschreibung

Immunogen: native purified 33 kDa protein from Spinacia oleracea

Format: Lyophilized

Beschreibung: PSII reaction centre components are generating the redox potential required todrive highly oxidizing water splitting reaction. Four Mn atoms are present on alumenal surface and form the catalyctic site of the water-splitting reactionwhich is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa(PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsicprotein is also termed the Mn-stabilizing protein (MSP), however recent evidencesshown that it is C-terminal domain of PsbA (D1) protein which is involved in in theassembly and stabilization of the OEC.

Spezifität: Predicted reactivity: dicots including: Nicotiana tabacum, Solanum lycopersicum, trees: Populustrichocarpa.

Anwendungen

Applikationshinweise: Recommended Dilution 1:2000-1: 5000 with standard ECL (WB).

Reinigung: total IgG fraction has been purified by 40% ammonium sulpgate precipitationfollowed by DEAE cellulose chromatography

Buffer: total IgG in PBS pH 7.4

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942 (2 ug of total cellular protein was loaded per lane)