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TNF alpha Antikörper (AA 80-235)

TNF alpha Reaktivität: Ratte WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1078627
  • Target Alle TNF alpha Antikörper anzeigen
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Bindungsspezifität
    • 47
    • 32
    • 15
    • 15
    • 14
    • 13
    • 10
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 80-235
    Reaktivität
    • 334
    • 203
    • 129
    • 67
    • 51
    • 42
    • 27
    • 26
    • 23
    • 16
    • 12
    • 10
    • 10
    • 9
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Ratte
    Wirt
    • 262
    • 204
    • 46
    • 26
    • 6
    • 3
    • 2
    • 2
    • 2
    Kaninchen
    Klonalität
    • 279
    • 265
    Polyklonal
    Konjugat
    • 261
    • 89
    • 45
    • 20
    • 19
    • 13
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TNF alpha Antikörper ist unkonjugiert
    Applikation
    • 355
    • 218
    • 140
    • 131
    • 74
    • 64
    • 58
    • 54
    • 49
    • 42
    • 40
    • 38
    • 37
    • 32
    • 20
    • 12
    • 10
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Verwendungszweck
    Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
    Spezifität
    The antibody is a rabbit polyclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
    Kreuzreaktivität
    Maus
    Aufreinigung
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Leu80~Leu235 with N-terminal His Tag
    Isotyp
    IgG
    Top Product
    Discover our top product TNF alpha Primärantikörper
  • Applikationshinweise

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    24 months
  • Parida, Das, Raju, Sathyaprabha: "Human placental extract ameliorates cytokine and cytokine receptor signaling in the rat hippocampus upon Benzo[a]Pyrene exposure." in: Journal of chemical neuroanatomy, Vol. 98, pp. 8-16, (2020) (PubMed).

    Chu, Zheng, Li, Zhang, Zhang, Xu, Chen: "Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-? and NF-?B." in: Experimental and therapeutic medicine, Vol. 5, Issue 2, pp. 423-428, (2013) (PubMed).

    Wang, Zheng, Liang, Zhao, Qian, Liang, Feng: "Hepatocellular necrosis, apoptosis, and proliferation after transcatheter arterial embolization or chemoembolization in a standardized rabbit model." in: Journal of vascular and interventional radiology : JVIR, Vol. 22, Issue 11, pp. 1606-12, (2011) (PubMed).

    Volentine, Ogden, Kortje, Tuma, Sorrell: "Role of acetaldehyde in the ethanol-induced impairment of hepatic glycoprotein secretion in the rat in vivo." in: Hepatology (Baltimore, Md.), Vol. 7, Issue 3, pp. 490-5, (1987) (PubMed).

  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Andere Bezeichnung
    Tumor Necrosis Factor Alpha (TNF alpha Produkte)
    Synonyme
    DIF antikoerper, TNF-alpha antikoerper, TNFA antikoerper, TNFSF2 antikoerper, RATTNF antikoerper, Tnfa antikoerper, tnf antikoerper, TNF-a antikoerper, TNFalpha antikoerper, Tnfsf1a antikoerper, TNFa antikoerper, cTNF antikoerper, Tnf-alpha antikoerper, tnfa-like antikoerper, TNF-ALPHA antikoerper, dif antikoerper, tnfa antikoerper, xtnf antikoerper, tnfsf2 antikoerper, tnf-alpha antikoerper, Cachectin antikoerper, tumor necrosis factor antikoerper, tumor necrosis factor b (TNF superfamily, member 2) antikoerper, tumor necrosis factor alpha antikoerper, tumor necrosis factor a (TNF superfamily, member 2) antikoerper, TNF antikoerper, Tnf antikoerper, tnf antikoerper, tnfb antikoerper, tnf-alpha antikoerper, LOC103694380 antikoerper, tnfa antikoerper
    Hintergrund
    DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
    Pathways
    NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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