Kaninchen anti-Human Ig (Chain kappa), (Light Chain) Antikörper

Details zu Produkt Nr. ABIN1687990, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Chain kappa, Light Chain
537
390
289
66
25
16
13
10
7
6
5
3
2
2
1
Reaktivität
Human
678
213
74
30
19
15
10
6
5
5
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Wirt
Kaninchen
399
392
157
78
21
7
4
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Free kappa light chain from pooled human IgG lambda myeloma sera.
Klon SP148
Isotyp IgG
Produktmerkmale Cellular Localization: Cytoplasm
Reinigung Purified by Protein A/G
Andere Bezeichnung Immunoglobulin Ig
Hintergrund Kappa light chains are polypeptide chains resided in the cell membrane and cytoplasmic regions of normal B cells and plasma cells. The combination of kappa light chains and heavy chains forms immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains. Individual lymphoid cell only contains a single light chain class. This clonal restriction may be used to indicate the polyclonal or monoclonal nature of B cell and plasma cell populations.The ratio of kappa to lambda is about 2:1. The level of kappa or lambda can be greatly elevated in serum of patients with multiple myeloma or other B cell malignancies.
Molekulargewicht 25 kDa
Gen-ID 3514
Forschungsgebiet Secondary Antibodies
Applikationshinweise IHC Dilution: 1:100
Kommentare

Positive Control:
IHC (p): Tonsil
WB: Spleen lysate

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C
Bilder des Herstellers
Western Blotting (WB) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 2)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 3)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 4)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 5)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 6)
Immunohistochemistry (IHC) image for Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (ABIN1687990) Rabbit anti-Human Ig (Chain kappa), (Light Chain) antibody (Image 7)