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CD4 Antikörper (CD4 Molecule) (Internal Region)

Details für Produkt anti-CD4 Antikörper Nr. ABIN1687412, Anbieter: Anmelden zum Anzeigen
Antigen
Alternativen
anti-Human CD4 Antikörper für Flow Cytometry
Epitop
Internal Region
67
22
20
13
13
13
10
8
8
7
7
6
6
6
5
5
5
5
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4
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3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
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Reaktivität
Human
970
616
215
64
42
29
24
21
20
15
13
9
8
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7
6
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5
3
3
2
2
2
2
1
1
Wirt
Kaninchen
1156
576
190
4
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD4 Antikörper ist unkonjugiert
265
225
159
115
38
36
26
24
21
20
19
16
15
15
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13
10
8
8
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5
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2
1
1
1
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Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1447
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331
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94
86
64
33
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2
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Hersteller
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Immunogen Synthetic peptide corresponding to internal region of human CD4.
Klon SP35
Isotyp IgG
Homologie Pig (Porcine)
Produktmerkmale Cellular Localization: Membrane
Reinigung Purified by Protein A/G
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Hintergrund CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T cell lymphomas. Non-specific nuclear staining is sometimes seen in follicles with this antibody, however in most cases it does not interfere with the interpretation of the CD4 membrane staining.
Molekulargewicht 59 kDa
Gen-ID 920
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Applikationshinweise IHC Dilution: 1:50
Kommentare

Positive Control:
IHC (p): Tonsil, T Cell Lymphoma
FC: Jurkat cell line

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody in Jurkats (green) compar...
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 4)
Produkt verwendet in: Mlecnik, Bindea, Kirilovsky et al.: "The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis." in: Science translational medicine, Vol. 8, Issue 327, pp. 327ra26, 2016 (PubMed).

David, Huang: "Diagnostic Utility of Flow Cytometry Analysis of Reactive T Cells in Nodular Lymphocyte-Predominant Hodgkin Lymphoma." in: American journal of clinical pathology, Vol. 145, Issue 1, pp. 107-15, 2015 (PubMed).

Baba, Kagoya, Kondo et al.: "T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients." in: Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, Vol. 11, pp. 33, 2015 (PubMed).

Nagata, Kontani, Enami et al.: "Variegated RHOA mutations in adult T-cell leukemia/lymphoma. ..." in: Blood, Vol. 127, Issue 5, pp. 596-604, 2016 (PubMed).

Ravanidis, Poulatsidou, Lagoudaki et al.: "Subcutaneous Transplantation of Neural Precursor Cells in Experimental Autoimmune Encephalomyelitis Reduces Chemotactic Signals in the Central Nervous System." in: Stem cells translational medicine, Vol. 4, Issue 12, pp. 1450-62, 2015 (PubMed).

Qin, Wang, Liu et al.: "Prognosis of nonspecific interstitial pneumonia correlates with perivascular CD4+ T lymphocyte infiltration of the lung." in: BMC pulmonary medicine, Vol. 15, pp. 127, 2015 (PubMed).

Szablewski, René, Costes: "Indolent cytotoxic T cell lymphoproliferation associated with nodular regenerative hyperplasia: a common liver lesion in the context of common variable immunodeficiency disorder." in: Virchows Archiv : an international journal of pathology, 2015 (PubMed).

Sanmamed, Rodriguez, Schalper et al.: "Nivolumab and Urelumab Enhance Antitumor Activity of Human T Lymphocytes Engrafted in Rag2-/-IL2Rγnull Immunodeficient Mice." in: Cancer research, Vol. 75, Issue 17, pp. 3466-78, 2015 (PubMed).

Guislain, Gadiot, Kaiser et al.: "Sunitinib pretreatment improves tumor-infiltrating lymphocyte expansion by reduction in intratumoral content of myeloid-derived suppressor cells in human renal cell carcinoma." in: Cancer immunology, immunotherapy : CII, Vol. 64, Issue 10, pp. 1241-50, 2015 (PubMed).

Llosa, Cruise, Tam et al.: "The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints." in: Cancer discovery, Vol. 5, Issue 1, pp. 43-51, 2015 (PubMed).

Lemos, Lama, Karuna et al.: "The inner foreskin of healthy males at risk of HIV infection harbors epithelial CD4+ CCR5+ cells and has features of an inflamed epidermal barrier." in: PLoS ONE, Vol. 9, Issue 9, pp. e108954, 2014 (PubMed).

Carulli, Ottaviano, Guerri et al.: "Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-lymphocyte clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas." in: Hematology reports, Vol. 6, Issue 2, pp. 5381, 2014 (PubMed).

Cogbill, Swerdlow, Gibson: "Utility of CD279/PD-1 immunohistochemistry in the evaluation of benign and neoplastic T-cell-rich bone marrow infiltrates." in: American journal of clinical pathology, Vol. 142, Issue 1, pp. 88-98, 2014 (PubMed).

Liu, De Marchi, Timmermans et al.: "Ferritin heavy chain in triple negative breast cancer: a favorable prognostic marker that relates to a cluster of differentiation 8 positive (CD8+) effector T-cell response." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 7, pp. 1814-27, 2014 (PubMed).

Mori, Hiroi, Shimada et al.: "Infiltration of m2 tumor-associated macrophages in oral squamous cell carcinoma correlates with tumor malignancy." in: Cancers, Vol. 3, Issue 4, pp. 3726-39, 2013 (PubMed).

Quintanilla-Martinez, Ridaura, Nagl et al.: "Hydroa vacciniforme-like lymphoma: a chronic EBV+ lymphoproliferative disorder with risk to develop a systemic lymphoma." in: Blood, Vol. 122, Issue 18, pp. 3101-10, 2013 (PubMed).

Yoshii, Ishida, Hodohara et al.: "Systemic Epstein-Barr virus-positive T-cell lymphoproliferative disease of childhood: Report of a case with review of the literature." in: Oncology letters, Vol. 4, Issue 3, pp. 381-384, 2013 (PubMed).

Cimino-Mathews, Ye, Meeker et al.: "Metastatic triple-negative breast cancers at first relapse have fewer tumor-infiltrating lymphocytes than their matched primary breast tumors: a pilot study." in: Human pathology, Vol. 44, Issue 10, pp. 2055-63, 2013 (PubMed).

Ishida, Mochizuki, Saito et al.: "CD8(+) mycosis fungoides with esophageal involvement: A case report." in: Oncology letters, Vol. 5, Issue 1, pp. 73-75, 2012 (PubMed).

Rigau, Mania, Béfort et al.: "Lethal multiple sclerosis relapse after natalizumab withdrawal." in: Neurology, Vol. 79, Issue 22, pp. 2214-6, 2012 (PubMed).

Donson, Birks, Schittone et al.: "Increased immune gene expression and immune cell infiltration in high-grade astrocytoma distinguish long-term from short-term survivors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 4, pp. 1920-7, 2012 (PubMed).