CD4 Antikörper (CD4 Molecule) (Internal Region)

Details for Product anti-CD4 Antibody No. ABIN1687412, Anbieter: Anmelden zum Anzeigen
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternativen
anti-Human CD4 Antikörper für Activation
Epitop
Internal Region
66
64
21
18
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1219
632
234
69
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Wirt
Kaninchen
1379
587
280
10
4
1
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD4 Antikörper ist unkonjugiert
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1744
490
442
354
321
310
157
155
87
78
39
38
30
30
25
19
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Immunogen Synthetic peptide corresponding to internal region of human CD4.
Klon SP35
Isotyp IgG
Homologie Pig (Porcine)
Produktmerkmale Cellular Localization: Membrane
Reinigung Purified by Protein A/G
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Hintergrund CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T cell lymphomas. Non-specific nuclear staining is sometimes seen in follicles with this antibody, however in most cases it does not interfere with the interpretation of the CD4 membrane staining.
Molekulargewicht 59 kDa
Gen-ID 920
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Applikationshinweise IHC Dilution: 1:50
Kommentare

Positive Control:
IHC (p): Tonsil, T Cell Lymphoma
FC: Jurkat cell line

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody in Jurkats (green) compar...
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 4)
Produkt verwendet in: Nagata, Kontani, Enami, Kataoka, Ishii, Totoki, Kataoka, Hirata, Aoki, Nakano, Kitanaka, Sakata-Yanagimoto, Egami, Shiraishi, Chiba, Tanaka, Shiozawa, Yoshizato, Suzuki, Kon, Yoshida, Sato et al.: "Variegated RHOA mutations in adult T-cell leukemia/lymphoma. ..." in: Blood, Vol. 127, Issue 5, pp. 596-604, 2016 (PubMed).

Mlecnik, Bindea, Kirilovsky, Angell, Obenauf, Tosolini, Church, Maby, Vasaturo, Angelova, Fredriksen, Mauger, Waldner, Berger, Speicher, Pagès, Valge-Archer, Galon: "The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis." in: Science translational medicine, Vol. 8, Issue 327, pp. 327ra26, 2016 (PubMed).

Lavotshkin, Jalas, Torisu-Itakura, Ozao-Choy, Lee, Sim, Stojadinovic, Wainberg, Bifulco, Fox, Bilchik: "Immunoprofiling for prognostic assessment of colon cancer: a novel complement to ultrastaging." in: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, Vol. 19, Issue 6, pp. 999-1006, 2015 (PubMed).

Nishikori, Kitano, Kobayashi, Hishizawa, Kitawaki, Kondo, Yamashita, Kawabata, Kadowaki, Takei, Haga, Takaori-Kondo: "Increased number of peripheral CD8+ T cells but not eosinophils is associated with late-onset skin reactions caused by bendamustine." in: International journal of hematology, Vol. 102, Issue 1, pp. 53-8, 2015 (PubMed).

Galgani, Insabato, Calì, Della Gatta, Mirra, Papaccio, Santopaolo, Alviggi, Mollo, Strina, Matarese, Beguinot, De Placido, Ulianich: "Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity." in: Fertility and sterility, Vol. 103, Issue 6, pp. 1579-86.e1, 2015 (PubMed).

Pino, Erkizia, Benet, Erikson, Fernández-Figueras, Guerrero, Dalmau, Ouchi, Rausell, Ciuffi, Keppler, Telenti, Kräusslich, Martinez-Picado, Izquierdo-Useros: "HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells." in: Retrovirology, Vol. 12, pp. 37, 2015 (PubMed).

Zanelli, Valli, Capodanno, Ragazzi, Ascani: "Anaplastic lymphoma kinase-positive large B-cell lymphoma: description of a case with an unexpected clinical outcome." in: International journal of surgical pathology, Vol. 23, Issue 1, pp. 78-83, 2015 (PubMed).

Brown, Ross, Gudjonsson, Wale, Pawarode, Maillard, Hristov: "Subcutaneous panniculitis-like T-cell lymphoma with bone marrow involvement." in: American journal of clinical pathology, Vol. 143, Issue 2, pp. 265-73, 2015 (PubMed).

Llosa, Cruise, Tam, Wicks, Hechenbleikner, Taube, Blosser, Fan, Wang, Luber, Zhang, Papadopoulos, Kinzler, Vogelstein, Sears, Anders, Pardoll, Housseau: "The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints." in: Cancer discovery, Vol. 5, Issue 1, pp. 43-51, 2015 (PubMed).

Guislain, Gadiot, Kaiser, Jordanova, Broeks, Sanders, van Boven, de Gruijl, Haanen, Bex, Blank: "Sunitinib pretreatment improves tumor-infiltrating lymphocyte expansion by reduction in intratumoral content of myeloid-derived suppressor cells in human renal cell carcinoma." in: Cancer immunology, immunotherapy : CII, Vol. 64, Issue 10, pp. 1241-50, 2015 (PubMed).

Baba, Kagoya, Kondo, Suzukawa, Ohta, Yamasoba: "T-cell phenotypes in chronic rhinosinusitis with nasal polyps in Japanese patients." in: Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, Vol. 11, pp. 33, 2015 (PubMed).

David, Huang: "Diagnostic Utility of Flow Cytometry Analysis of Reactive T Cells in Nodular Lymphocyte-Predominant Hodgkin Lymphoma." in: American journal of clinical pathology, Vol. 145, Issue 1, pp. 107-15, 2015 (PubMed).

Qin, Wang, Liu, Xiao, Qin, Zheng, Shi: "Prognosis of nonspecific interstitial pneumonia correlates with perivascular CD4+ T lymphocyte infiltration of the lung." in: BMC pulmonary medicine, Vol. 15, pp. 127, 2015 (PubMed).

Ravanidis, Poulatsidou, Lagoudaki, Touloumi, Polyzoidou, Lourbopoulos, Nousiopoulou, Theotokis, Kesidou, Tsalikakis, Karacostas, Grigoriou, Chlichlia, Grigoriadis: "Subcutaneous Transplantation of Neural Precursor Cells in Experimental Autoimmune Encephalomyelitis Reduces Chemotactic Signals in the Central Nervous System." in: Stem cells translational medicine, Vol. 4, Issue 12, pp. 1450-62, 2015 (PubMed).

Sanmamed, Rodriguez, Schalper, Oñate, Azpilikueta, Rodriguez-Ruiz, Morales-Kastresana, Labiano, Pérez-Gracia, Martín-Algarra, Alfaro, Mazzolini, Sarno, Hidalgo, Korman, Jure-Kunkel, Melero: "Nivolumab and Urelumab Enhance Antitumor Activity of Human T Lymphocytes Engrafted in Rag2-/-IL2Rγnull Immunodeficient Mice." in: Cancer research, Vol. 75, Issue 17, pp. 3466-78, 2015 (PubMed).

Szablewski, René, Costes: "Indolent cytotoxic T cell lymphoproliferation associated with nodular regenerative hyperplasia: a common liver lesion in the context of common variable immunodeficiency disorder." in: Virchows Archiv : an international journal of pathology, 2015 (PubMed).

Wick, Webb, Nielsen, Martin, Kroeger, Milne, Castellarin, Twumasi-Boateng, Watson, Holt, Nelson: "Surveillance of the tumor mutanome by T cells during progression from primary to recurrent ovarian cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 5, pp. 1125-34, 2014 (PubMed).

Kragsnaes, Fredberg, Stribolt, Kjaer, Bendix, Ellingsen: "Stereological quantification of immune-competent cells in baseline biopsy specimens from achilles tendons: results from patients with chronic tendinopathy followed for more than 4 years." in: The American journal of sports medicine, Vol. 42, Issue 10, pp. 2435-45, 2014 (PubMed).

Carulli, Ottaviano, Guerri, Giuntini, Sammuri, Ciancia, Azzarà: "Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-lymphocyte clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas." in: Hematology reports, Vol. 6, Issue 2, pp. 5381, 2014 (PubMed).

Liu, De Marchi, Timmermans, Beekhof, Trapman-Jansen, Foekens, Look, van Deurzen, Span, Sweep, Brask, Timmermans-Wielenga, Debets, Martens, Foekens, Umar: "Ferritin heavy chain in triple negative breast cancer: a favorable prognostic marker that relates to a cluster of differentiation 8 positive (CD8+) effector T-cell response." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 7, pp. 1814-27, 2014 (PubMed).