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CD4 Antikörper (CD4 Molecule) (Internal Region)

Details für Produkt anti-CD4 Antikörper Nr. ABIN1687412, Anbieter: Anmelden zum Anzeigen
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternativen
anti-Human CD4 Antikörper für Flow Cytometry
Epitop
Internal Region
66
65
22
17
15
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15
12
10
8
8
8
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5
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2
2
2
2
2
1
1
1
1
1
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Reaktivität
Human
1189
667
252
72
61
43
34
24
22
22
19
17
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11
11
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10
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5
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2
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2
1
1
1
1
Wirt
Kaninchen
1359
627
272
9
4
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD4 Antikörper ist unkonjugiert
281
239
171
106
47
45
43
36
36
35
34
32
25
25
21
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20
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13
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Applikation
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1755
468
442
330
314
298
151
150
85
61
39
38
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14
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1
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Optionen
Hersteller
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Immunogen Synthetic peptide corresponding to internal region of human CD4.
Klon SP35
Isotyp IgG
Homologie Pig (Porcine)
Produktmerkmale Cellular Localization: Membrane
Reinigung Purified by Protein A/G
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Hintergrund CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T cell lymphomas. Non-specific nuclear staining is sometimes seen in follicles with this antibody, however in most cases it does not interfere with the interpretation of the CD4 membrane staining.
Molekulargewicht 59 kDa
Gen-ID 920
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Applikationshinweise IHC Dilution: 1:50
Kommentare

Positive Control:
IHC (p): Tonsil, T Cell Lymphoma
FC: Jurkat cell line

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) Flow cytometric analysis of rabbit anti-CD4 (SP35) antibody in Jurkats (green) compar...
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-CD4 Antikörper (CD4 Molecule) (Internal Region) (ABIN1687412) anti-CD4 Molecule (CD4) (Internal Region) antibody (Image 4)
Produkt verwendet in: Nagata, Kontani, Enami, Kataoka, Ishii, Totoki, Kataoka, Hirata, Aoki, Nakano, Kitanaka, Sakata-Yanagimoto, Egami, Shiraishi, Chiba, Tanaka, Shiozawa, Yoshizato, Suzuki, Kon, Yoshida, Sato et al.: "Variegated RHOA mutations in adult T-cell leukemia/lymphoma. ..." in: Blood, Vol. 127, Issue 5, pp. 596-604, 2016 (PubMed).

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Galgani, Insabato, Calì, Della Gatta, Mirra, Papaccio, Santopaolo, Alviggi, Mollo, Strina, Matarese, Beguinot, De Placido, Ulianich: "Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity." in: Fertility and sterility, Vol. 103, Issue 6, pp. 1579-86.e1, 2015 (PubMed).

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Llosa, Cruise, Tam, Wicks, Hechenbleikner, Taube, Blosser, Fan, Wang, Luber, Zhang, Papadopoulos, Kinzler, Vogelstein, Sears, Anders, Pardoll, Housseau: "The vigorous immune microenvironment of microsatellite instable colon cancer is balanced by multiple counter-inhibitory checkpoints." in: Cancer discovery, Vol. 5, Issue 1, pp. 43-51, 2015 (PubMed).

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David, Huang: "Diagnostic Utility of Flow Cytometry Analysis of Reactive T Cells in Nodular Lymphocyte-Predominant Hodgkin Lymphoma." in: American journal of clinical pathology, Vol. 145, Issue 1, pp. 107-15, 2015 (PubMed).

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Szablewski, René, Costes: "Indolent cytotoxic T cell lymphoproliferation associated with nodular regenerative hyperplasia: a common liver lesion in the context of common variable immunodeficiency disorder." in: Virchows Archiv : an international journal of pathology, 2015 (PubMed).

Wick, Webb, Nielsen, Martin, Kroeger, Milne, Castellarin, Twumasi-Boateng, Watson, Holt, Nelson: "Surveillance of the tumor mutanome by T cells during progression from primary to recurrent ovarian cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 5, pp. 1125-34, 2014 (PubMed).

Kragsnaes, Fredberg, Stribolt, Kjaer, Bendix, Ellingsen: "Stereological quantification of immune-competent cells in baseline biopsy specimens from achilles tendons: results from patients with chronic tendinopathy followed for more than 4 years." in: The American journal of sports medicine, Vol. 42, Issue 10, pp. 2435-45, 2014 (PubMed).

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