Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (C-Term) Antikörper

Details zu Produkt Nr. ABIN1686882, Anbieter: Anmelden zum Anzeigen
Antigen
  • ID3
  • MOR136-6
  • fa56g03
  • tdsubc_2g3
  • wu:fa56g03
  • ik:tdsubc_2g3
  • xx:tdsubc_2g3
  • heir-1
  • xid3
  • xidia
  • Hlh462
  • Idb3
  • bHLHb25
  • HEIR-1
  • inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
  • olfactory receptor 50
  • inhibitor of DNA binding 3
  • ID3
  • Olfr50
  • id3
  • Id3
Epitop
C-Term
18
12
10
8
8
8
7
5
5
3
2
2
1
Reaktivität
Human
140
60
27
7
6
6
5
4
4
3
3
2
Wirt
Kaninchen
73
69
Klonalität
Polyklonal
Konjugat
Unkonjugiert
6
6
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
108
49
43
23
21
19
18
18
13
4
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide corresponding to C-terminus of human ID-3.
Isotyp IgG
Produktmerkmale Cellular Localization: Nucleus
Reinigung Immunogen Affinity Purified
Andere Bezeichnung ID3 (ID3 Antibody Abstract)
Hintergrund Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E2A complex, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.
Molekulargewicht 14 kDa
Gen-ID 3399
Forschungsgebiet Stem Cells, Transcription Factors, Adaptive Immunity, Apoptosis/Necrosis
Applikationshinweise IHC Dilution: Ready to use, for IHC only
Kommentare

Positive Control:
IHC (p): Breast carcinoma

Protokoll IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Lagerung 4 °C
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (C-Term) antibody (ABIN1686882) anti-Inhibitor of DNA Binding 3, Dominant Negative Helix-Loop-Helix Protein (ID3) (C-Term) antibody
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