WD Repeat and HMG-Box DNA Binding Protein 1 (WDHD1) Antikörper

Antigen: WD Repeat and HMG-Box DNA Binding Protein 1 (WDHD1)

Synonyme: AND-1, A630092E18, D630024B06Rik, wdhd1, MGC130701, zgc:92585, wu:fa11f10, wu:fb77d12, WDHD1

Klonalität: Monoklonal (20G10)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Western Blot (WB)

Produktnummer: ABIN161176

Produktbeschreibung

Weitere Bezeichnung: AND-1 (WDHD1)

Immunogen: Recombinant (partial), C-terminal

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 20G10

Beschreibung: The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be in chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting. Distribution: Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm. Function: Binds DNA with high affinity, may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication. Modifications: Phosphorylation (S1041) Synonyms: WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1 Structure: Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Jurkat cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-AND-1 (Clone 20G10) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Publikationen

Publikationen: Koehler, Schmidt-Zachmann, Franke: "AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats." in: Journal of cell science, Vol. 110 ( Pt 9), pp. 1051-62, 1997 (PubMed).