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Antikörper
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anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor,...
Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) Antikörper
| Antigen |
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| Synonyme |
IKBA, MAD-3, NFKBI |
| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)
Western Blot (WB) (105), Immunpräzipitation (IP) (35), Immunhistochemie (Paraffinschnitte) (IHC (p)) (29), Immunhistochemie (IHC) (27), Enzyme Immunoassay (EIA) (25), Immunfluoreszenz (IF) (23), ELISA (13), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (13), Durchflusszytometrie (FACS) (3), Dot Blot (Dot) (2), Elektromobilitäts-Experimente (EMSA) (2), Gel Shift (GS) (2), Immunzytochemie (ICC) (2)
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| Produktnummer |
ABIN161060 |
| Menge |
200 µl (20 WB) (Varianten) |
| Preis |
Produkt für diese Region nicht verfügbar.
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| Lieferung nach |
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Weitere Bezeichnung
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IkappaB-alpha
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Immunogen
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Peptide mapping to the carboxy terminus of human IkappaB-alpha
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Format
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Purified
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Isotyp
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IgG (Passende Sekundärantikörper)
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Klon
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Poly6249
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Beschreibung
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IkappaB-alpha is an inhibitory protein that binds the NFkappaB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IkappaB proteins are phosphorylated and degraded via the ubiquitin pathway allowing nuclear translocation of NFkappaB. The NFkappaB complex binds DNA to activate gene expression of a number of a variety of proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis. Poly6249 recognizes the C-terminal region of human, mouse, rat IKB-alpha and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry. Distribution: Cytoplasm Function: Regulates transcriptional activity of NFkappaB by binding complex and trapping in the cytoplasm. Serine phosphorylation of IkappaB proteins targets them for destruction via the ubiquitination pathway and allows the NFkappaB complex to translocate to nucleus and bind DNA to activate transcription. Modifications: Phosphorylation, ubiquitination, sumoylation Synonyms: Nuclear factor of kappa light chain enhancer in B cells inhibitor alpha, IKBA Structure: Inhibitory protein for NFkappaB complex, contains multiple ankyrin repeats, approximately 39 kD.
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Spezifität
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NFkB1, SUMO4, p53, ribosomal S6 kinase 1, and many others.
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Applikationshinweise
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
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Reinigung
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This antibody was purified by antigen-affinity chromatography.
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Buffer
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This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
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Lagerung
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The antibody solution should be stored undiluted at 4°C. DO NOT FREEZE.
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Forschungsgebiet
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Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", Typ "Antikörper" finden
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Wirte
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Kaninchen (92), Maus (28), Schaf (5)
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Reaktivitäten
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Human (115), Maus (61), Ratte (Rattus) (51), Schwein (4), Rind (Kuh) (3), Hund (3), Huhn (2), Schaf (1)
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Applikationen
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Western Blot (WB) (105), Immunpräzipitation (IP) (35), Immunhistochemie (Paraffinschnitte) (IHC (p)) (29), Immunhistochemie (IHC) (27), Enzyme Immunoassay (EIA) (25), Immunfluoreszenz (IF) (23), ELISA (13), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (13), Durchflusszytometrie (FACS) (3), Dot Blot (Dot) (2), Elektromobilitäts-Experimente (EMSA) (2), Gel Shift (GS) (2), Immunzytochemie (ICC) (2)
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Konjugate
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Biotin (3)
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Epitope
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pSer32/36 (8), pTyr42 (4), C-Term (2), pSer32, pSer36 (2), AA 32-291 (1), Sumoylation Site (1), Y181 (1), pTyr305 (1)
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