NF kappa B P65 Antikörper

Antigen: NF kappa B P65

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus), Human

Applikation: Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)

Produktnummer: ABIN161023

Produktbeschreibung

Weitere Bezeichnung: NF-kappaB p65

Immunogen: peptide-KLH, C-terminal

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6226

Beschreibung: NF-kappaB/p65 (nuclear factor kappa light chain enhancer in B cells p65) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-kappaB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-kappaB/p65 is bound (with p50) to IkappaB inhibitor in cytoplasm in inactive form. Phosphorylation of IkappaB releases and NF-kappaB which goes to the nucleus to activate gene expression. NF-kappaB can be activated with LPS, TNF-alpha, phorbol ester, or IL-1. The Poly6226 antibody reacts with the C-terminal region of human, mouse, and rat NF-kappaB p50 and has been shown to be useful for Western blotting. Distribution: Ubiquitously expressed nuclear protein Function: Transcriptional activator, originally identified as an activator of kappa light chain in B cells Synonyms: NFkappaB p65, nuclear factor kappa light chain enhancer in B cells p65 Regulation: Bound (with p50) to IkB inhibitor in cytoplasm in inactive form. Phosphorylation of IkB releases and NF-kB goes to the nucleus to activate gene expression. Stimulation with LPS, TNF-alpha, phorbol ester, or IL-1 activates Structure: Member of the Rel/dorsal family. One subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits derived from larger precursor proteins

Spezifität: Interacts with NF-kappaB p50 and IkappaB.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.

Lagerung: Upon receipt, store undiluted at 4° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-NF-kappaB p65 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Publikationen

Publikationen: Leitch, Barrans, Jack et al.: "Dysregulation of apoptosis in Waldenstrom's macroglobulinemia does not involve nuclear factor kappa B activation." in: Seminars in oncology, Vol. 30, Issue 2, pp. 161-4, 2003 (PubMed).

Stjernholm-Vladic, Stygar, Mansson et al.: "Factors involved in the inflammatory events of cervical ripening in humans." in: Reproductive biology and endocrinology : RB&E, Vol. 2, pp. 74, 2004 (PubMed).

Popov, Patsenker, Bauer et al.: "Halofuginone induces matrix metalloproteinases in rat hepatic stellate cells via activation of p38 and NFkappaB." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15090-8, 2006 (PubMed).

Zuo, Xue, Chen: "14-3-3 Eepsilon Dynamically Interacts with Key Components of MAPK Signal Module for Selective Modulation of the TNF--induced Time Course-dependent NF-B Activity." in: Journal of proteome research, 2010 (PubMed).