Actin, beta (ACTB) Antikörper

Antigen: Actin, beta (ACTB)

Synonyme: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Ratte (Rattus), Human

Applikation: Western Blot (WB)

Produktnummer: ABIN161018

Produktbeschreibung

Weitere Bezeichnung: beta-actin

Immunogen: Peptide-KLH, NH2 terminus

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6221

Beschreibung: Beta-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human beta-actin. Distribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells Function: Actins are highly conserved proteins that are involved in cell motility Synonyms: Actin, cytoplasmic 1 Regulation: Three actin isoforms exist in vertebrates, alpha, found in muscle tissues, beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network. Structure: Member of the actin family, 42 KD

Spezifität: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.

Synonyme: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-beta-actin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Publikationen

Publikationen: Joyce, Wagner, Basso et al.: "ABCA1 overexpression in the liver of LDLr-KO mice leads to accumulation of pro-atherogenic lipoproteins and enhanced atherosclerosis." in: The Journal of biological chemistry, Vol. 281, Issue 44, pp. 33053-65, 2006 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).