Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) Antikörper

Antigen: Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ)

Synonyme: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, adiponectin, APN, Acdc, apM1, 30kDa, adipo, Acrp30, ADIPOQ, MGC138982, ADN, MGC84292

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN161012

Produktbeschreibung

Weitere Bezeichnung: Adiponectin

Immunogen: Recombinant (partial)

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6215

Beschreibung: Adiponectin is a 28 kD is a complement 1q family member that is involved in the control of fat metabolism and insulin sensitivity. Adiponectin forms multimers, under non-reducing, non-heat denaturing conditions with approximate molecular weights of >300 kD, 167 kD, and 67 kD. Under reducing conditions with no heat, adiponectin is approximately 67 kD, under reducing conditions with heat, the adiponectin monomer has an apparent molecular weight of approximately 28 kD. Adiponectin can be modified by glycosylation and hydroxylation. Adiponectin is synthesized and secreted into plasma by adipocytes. Adiponectin has also been shown to act as a negative regulator of hematopoiesis and inflammation. In macrophages and endothelial cells adiponectin has been shown to downregulate TNF-induced adhesion molecules, and block NF-kappaB signaling though a cAMP-dependent mechanism. Decreased levels of adiponectin are associated with obesity insulin resistance and type 2 diabetes. IL-6 inhibits adiponectin production and secretion. Adiponectin has been shown to interact with collagen type I, III, and V. The Poly6215 antibody has been shown to be useful for Western blotting of human adiponectin. Distribution: Synthesized and secreted by adipocytes into plasma Function: Involved in the control of fat metabolism and insulin sensitivity. Negative regulator in hematopoiesis and immunity. Acts as an anti-inflammatory agent in macrophages and endothelial cells (downregulates TNF-induced adhesion molecules), blocks NF-kappaB signaling though a cAMP-dependent mechanism. Decreased levels of adiponectin are associated with obesity, insulin resistance, and type 2 diabetes Modifications: Glycosylation, Hydroxylation Synonyms: Adipocyte-specific secretory protein, gelatin-binding factor 28 kD Regulation: IL-6 inhibits adiponectin production and secretion in cultured adipocytes Structure: Complement 1q family member, contains carboxyl-terminal domain and amino-terminal collagenous domain. Forms multimers, under non-reducing, non-heat denaturing conditions, approximate molecular weights are >300 kD, 167 kD, and 67 kD. Under reducing conditions with no heat, adiponectin is approximately 67 kD, under reducing conditions with heat, adiponectin monomer is approximately 28 kD

Spezifität: Interacts with collagen type I, III, and V.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Recombinant human adiponectin (1 ug/lane) was resolved by SDS-PAGE electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-adiponectin antibody. The protein was visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.