Stathmin 1 (STMN1) (pSer16) Antikörper

Antigen: Stathmin 1 (STMN1)

Synonyme: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, FLJ32206, MGC138869, MGC138870, 19k, P18, P19, Pig, Smn, Op18, Pp17, Pp18, Pp19, Pr22, Lap18, MGC72884, prosolin, stmn1, MGC64272, STMN1, lag, smn, op18, pp17, pp19, pr22, lap18, MGC89828, stathmin, fj43c10, wu:fj38b07, wu:fj43c10, zgc:110159, MGC127518, cb959, MGC136942, wu:fb14e04, zgc:136942, LOC100217988

Epitope: pSer16

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN160994

Produktbeschreibung

Weitere Bezeichnung: Stathmin/Op18 Phosphorylated (Ser16)

Immunogen: Modified peptide

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6202

Beschreibung: Stathmin/Op18 is a 19 kD member of the Stathmin family. This protein is localized in the cytoplasm and becomes nuclear during the S/G2 phase of the cell cycle. Stathmin/Op18 acts as a microtubule destabilizer in mitotic spindle regulation and sequesters tubulin dimers into assembly-incompetent complexes. This protein participates as an intracellular relay integrating regulatory signals of the cellular environment and has been shown to be involved in megakaryocyte polyploidization. Stathmin/Op18 can be phosphorylated in response to heat, Ser62 phosphorylation reduces tubulin binding ability. This protein can also be modified by acetylation. Stathmin/Op18 has been shown to interact with tubulin, KIST, CaM kinase II and IV, Cdc2, MAPK, and Cdk1. The Poly6202 antibody recognizes human phosphorylated stathmin/Op18 (Ser16) and has been shown to be useful for Western blotting. Distribution: Cytoplasm, nuclear during S/G2 Function: Microtubule destabilizer, mitotic spindle regulation, sequesters tubulin dimers into assembly incompetent complexes, intracellular relay integrating regulatory signals of the cellular environment, megakaryocyte polyploidization Modifications: Phosphorylation, Acetylation Synonyms: Oncoprotein 18 Regulation: Heat-induced phosphorylation, Ser62 phosphorylation reduces tubulin binding ability Structure: Stathmin family, 19 kD

Spezifität: Reacts against ser16-phosphorylated stathmin/op18 Tubulin, KIST, CaM kinase II and IV, Cdc2, MAPK, Cdk1. reacts against ser16-phosphorylated stathmin/op18

Synonyme: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, FLJ32206, MGC138869, MGC138870, 19k, P18, P19, Pig, Smn, Op18, Pp17, Pp18, Pp19, Pr22, Lap18, MGC72884, prosolin, stmn1, MGC64272, STMN1, lag, smn, op18, pp17, pp19, pr22, lap18, MGC89828, stathmin, fj43c10, wu:fj38b07, wu:fj43c10, zgc:110159, MGC127518, cb959, MGC136942, wu:fb14e04, zgc:136942, LOC100217988

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hela cells were treated with 300 µM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole (lane 2) for an additional 18hrs and cell extract prepared, resolved by electrophoresis, and transferred to nitrocellulose. Nitrocellulose blots in Panel A were treated overnight with 100 U/ml calf intestinal phosphatase (CIP) and probed with rabbit antibodies reactive against phosphorylated Stathmin/Op18 (Ser16). Nitrocellulose blots that were not treated with CIP (Panel B) were probed with rabbit antibodies reactive against phosphorylated Stathmin/Op18 (Ser16). In both cases proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.