Adducin 1 (Alpha) (ADD1) Antikörper

Antigen: Adducin 1 (Alpha) (ADD1)

Synonyme: ADDA, MGC3339, MGC44427, AI256389, MGC124621, ADD1, cb787, bZ1H20.1, MGC158352, zgc:158352, DKFZp459A0431, add1-a, add2, adda, MGC69376

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus, Human

Applikation: Western Blot (WB)

Produktnummer: ABIN160954

Produktbeschreibung

Weitere Bezeichnung: alpha-Adducin

Immunogen: Recombinant (partial)

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6170

Beschreibung: The alpha-adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca/calmodulin and acts as a substrate for protein kinases A and C. alpha-addducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers alpha/beta, and alpha/gamma. The molecular weights for the various isoforms are alpha=120 kD, beta=110kD, gamma=80kD. This protein has been reported to promote the assembly of the spectrin-actin network. Polymorphisms in alpha-adducin are associated with cardiovascular disease and hypertension. alpha-adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, alpha-adducin can regulate membrane ruffling and cell motility. The Poly6170 antibody has been shown to be useful for Western blotting of mouse and human alpha-adducin protein. Distribution: Ubiquitously expressed cytoskeletal protein Function: Binds with high affinity to Ca/calmodulin, substrate for protein kinases A and C, promotes the assembly of spectrin-actin network. Polymorphisms associated with cardiovascular disease and hypertension Modifications: Phosphorylation Synonyms: ADD-1 Regulation: Phosphorylation by Rho-kinase regulates membrane ruffling, cell motility Structure: Aldolase class II family, adducin subfamily, heterodimerizes alpha/beta, alpha/gamma. Multiple isoforms alpha-120 kD, beta-110 kD, gamma-80kD

Spezifität: Calmodulin.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-alpha-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.