P130 Cas Antikörper

Antigen: P130 Cas

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN160905

Produktbeschreibung

Immunogen: Recombinant (partial), C-terminal

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Poly6139

Beschreibung: P130 Cas (Crk-associated substrate) is a 120-130 kD docking protein and a central coordinator for tyrosine-kinase-based signaling related to cell adhesion. p130 Cas has been implicated in induction of cell migration, growth factor stimulation, cytokine receptor engagement, cardiovascular development, and actin filament assembly. p130 Cas is localized to focal adhesions and stress fibers. When unphosporylated, localizes in the cytoplasm, phosphorylation by FAK induces movement to membrane. p130 Cas is dephosphorylated by PTP-PEST, dephosphorylation blocks cell migration. p130 Cas has been reported to interact with v-Crk, v-Src, nephrocystin, PTK2B, CrkII and to form complexes with Fak1, CrkL, and Lyn kinase and to heterodimerize with CasL. The Poly6139 antibody recognizes the C-terminal region of human p130 Cas and has been shown to be useful for Western blotting. Distribution: Focal adhesions, stress fibers, unphosphorylated localizes to cytoplasm, moves to membrane upon tyrosine phosphorylation Function: Docking protein, central coordinator for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration, growth factor stimulation, cytokine receptor engagement, cardiovascular development, actin filament assembly Modifications: Phosphorylation by Fak1 Synonyms: Crk-associated substrate (Cas, CRKAS) Regulation: Dephosphorylated by PTP-PEST, blocks cell migration Structure: CAS family, SH2-, SH3- domains, HLH motif, isoforms 130 kD, 120 kD

Spezifität: V-Crk, v-Src, nephrocystin, PTK2B, CrkII. Forms complexes with Fak1, CrkL, Lyn kinase, heterodimerizes with CasL.

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by antigen-affinity chromatography.

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-p130 Cas antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Publikationen

Publikationen: Sakai, Iwamatsu, Hirano et al.: "A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner." in: The EMBO journal, Vol. 13, Issue 16, pp. 3748-56, 1994 (PubMed).

Brinkman, van der Flier, Kok et al.: "BCAR1, a human homologue of the adapter protein p130Cas, and antiestrogen resistance in breast cancer cells." in: Journal of the National Cancer Institute, Vol. 92, Issue 2, pp. 112-20, 2000 (PubMed).

Wei, Yang, Zhang et al.: "Anchorage-independent phosphorylation of p130(Cas) protects lung adenocarcinoma cells from anoikis." in: Journal of cellular biochemistry, Vol. 87, Issue 4, pp. 439-49, 2002 (PubMed).

Zhang, He, Zhou et al.: "Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 27319-28, 2003 (PubMed).