H2A.X-Phosphorylated (Ser139) Antikörper

Antigen: H2A.X-Phosphorylated

Epitope: Ser139

Klonalität: Monoklonal (2F3)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN160891

Produktbeschreibung

Weitere Bezeichnung: H2A.X-Phosphorylated (Ser139)

Immunogen: Modified peptide (KATQAS*QEY)

Format: Purified

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: 2F3

Beschreibung: H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence. Distribution: Nuclear Function: Phosphorylated H2AX promotes DNA repair and maintains genomic stability. Important for recombination between immunoglobulin switch regions Modifications: Phosphorylation on Ser139 after double-stranded DNA breaks Synonyms: H2A.x, H2a/x, Histone 2A, Histone 2A.X Regulation: Synthesized in G1 and S-phase of cell cycle Structure: Basal histone, H2 histone family, 14 kD

Spezifität: Reacts with ser139-phosphorylated h2a.reacts with ser139-phosphorylated h2a.x

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by protein G affinity chromatography

Buffer: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.

Lagerung: Upon receipt, store frozen at -20° C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Untreated (Lane 1) and staurosporine-treated (Lane 2) Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-H2A.X (Ser 139) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.

Publikationen

Publikationen: Mannironi, Bonner, Hatch: "H2A.X. a histone isoprotein with a conserved C-terminal sequence, is encoded by a novel mRNA with both DNA replication type and polyA 3' processing signals." in: Nucleic acids research, Vol. 17, Issue 22, pp. 9113-26, 1990 (PubMed).

Celeste, Petersen, Romanienko et al.: "Genomic instability in mice lacking histone H2AX." in: Science (New York, N.Y.), Vol. 296, Issue 5569, pp. 922-7, 2002 (PubMed).

Bassing, Chua, Sekiguchi et al.: "Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 12, pp. 8173-8, 2002 (PubMed).

Reina-San-Martin, Difilippantonio, Hanitsch et al.: "H2AX is required for recombination between immunoglobulin switch regions but not for intra-switch region recombination or somatic hypermutation." in: The Journal of experimental medicine, Vol. 197, Issue 12, pp. 1767-78, 2003 (PubMed).