Fibroblast Growth Factor 2 (Basic) (FGF2) Antikörper

Antigen: Fibroblast Growth Factor 2 (Basic) (FGF2)

Synonyme: BFGF, FGFB, HBGF-2, bFGF, Fgf-2, FGF2, nudt6, fgf2, MGC52602, FGF-2, bfgf, fgfb, fgf-2, xfgf2, hbgh-2, HBGF2, LOC100227798, Fgfb

Klonalität: Monoklonal (JKFb-1)

Wirt: Maus

Reaktivität: Human

Applikation: ELISA (Fangantikörper), ELISPOT (Fangantikörper), Immunhistochemie (IHC)

Produktnummer: ABIN160577

Produktbeschreibung

Weitere Bezeichnung: FGF-basic

Immunogen: Recombinant human bFGF

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: JKFb-1

Beschreibung: Modulator of cell proliferation, motility, differentiation, angiogenesis, affinity for heparin, associated with ECM Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-1 antibody reacts with human fibroblast growth factor - basic (FGF-basic). Synonyms: Fibroblast Growth Factor-basic, bFGF, FGF-2, Heparin-binding growth factor Regulation: Released after tissue injuries and during inflammatory processes Structure: FGF, 17.2 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA or ELISPOT Capture: The purified JKFb-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JKFb-2 antibody (ABIN160578) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections. Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4°C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Wachstumsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Fibroblast Growth Factor 2 (Basic) (FGF2) antibody

Publikationen

Publikationen: Klagsbrun: "Mediators of angiogenesis: the biological significance of basic fibroblast growth factor (bFGF)-heparin and heparan sulfate interactions." in: Seminars in cancer biology, Vol. 3, Issue 2, pp. 81-7, 1992 (PubMed).

Goldfarb: "The fibroblast growth factor family." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 1, Issue 9, pp. 439-45, 1991 (PubMed).

Sensenbrenner: "The neurotrophic activity of fibroblast growth factors." in: Progress in neurobiology, Vol. 41, Issue 6, pp. 683-704, 1994 (PubMed).