IL-17 Antikörper

Antigen: IL-17

Klonalität: Monoklonal (TC11-18H10-1)

Wirt: Ratte

Reaktivität: Maus

Applikation: ELISA (Fangantikörper), ELISPOT (Fangantikörper), Intrazelluläre Durchflusszytometrie (ICFC), Neutralisierung (Neut)

Produktnummer: ABIN160500

Produktbeschreibung

Immunogen: E. coli-expressed, recombinant mouse IL-17

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: TC11-18H10-1

Beschreibung: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells, stimulates cell migration, cord formation, and IL-6 secretion by stromal cells CD4 memory T cells IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-kappaB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-18H10.1 antibody can neutralize IL-17 activity. Synonyms: Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) Structure: Cytokine, dimer, 15 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA and ELISPOT Capture: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (ABIN160513) as the detecting antibody and recombinant mouse IL-17 (ABIN160666) as the standard. Flow Cytometry: The fluorochrome-labeled TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (ABIN160501). Additional reported applications (for the relevant formats) include: Western blotting. Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The immunoglobulin was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-17 antibody

Publikationen

Publikationen: Fossiez, Djossou, Chomarat et al.: "T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines." in: The Journal of experimental medicine, Vol. 183, Issue 6, pp. 2593-603, 1996 (PubMed).

Kennedy, Rossi, Zurawski et al.: "Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 16, Issue 8, pp. 611-7, 1997 (PubMed).

Yao, Spriggs, Derry et al.: "Molecular characterization of the human interleukin (IL)-17 receptor." in: Cytokine, Vol. 9, Issue 11, pp. 794-800, 1998 (PubMed).

Infante-Duarte, Horton, Byrne et al.: "Microbial lipopeptides induce the production of IL-17 in Th cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 11, pp. 6107-15, 2000 (PubMed).

Numasaki, Fukushi, Ono et al.: "Interleukin-17 promotes angiogenesis and tumor growth." in: Blood, Vol. 101, Issue 7, pp. 2620-7, 2003 (PubMed).

Schubert, Maier, Morawietz et al.: "Immunization with glucose-6-phosphate isomerase induces T cell-dependent peripheral polyarthritis in genetically unaltered mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4503-9, 2004 (PubMed).

Nekrasova, Shive, Gao et al.: "ERK1-deficient mice show normal T cell effector function and are highly susceptible to experimental autoimmune encephalomyelitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 4, pp. 2374-80, 2005 (PubMed).

Harrington, Hatton, Mangan et al.: "Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages." in: Nature immunology, Vol. 6, Issue 11, pp. 1123-32, 2005 (PubMed).

Hofstetter, Ibrahim, Koczan et al.: "Therapeutic efficacy of IL-17 neutralization in murine experimental autoimmune encephalomyelitis." in: Cellular immunology, Vol. 237, Issue 2, pp. 123-30, 2006 (PubMed).

Dong: "Diversification of T-helper-cell lineages: finding the family root of IL-17-producing cells." in: Nature reviews. Immunology, Vol. 6, Issue 4, pp. 329-33, 2006 (PubMed).

Yen, Cheung, Scheerens et al.: "IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6." in: The Journal of clinical investigation, Vol. 116, Issue 5, pp. 1310-6, 2006 (PubMed).

Ehirchiou, Xiong, Xu et al.: "CD11b facilitates the development of peripheral tolerance by suppressing Th17 differentiation." in: The Journal of experimental medicine, Vol. 204, Issue 7, pp. 1519-24, 2007 (PubMed).