GM-CSF Antikörper (Biotin)

Antigen: GM-CSF

Synonyme: GMCSF, GM-CSF

Klonalität: Monoklonal (MP1-31G6)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Biotin

Applikation: ELISA (Detektionsantikörper), ELISPOT (Detektionsantikörper)

Produktnummer: ABIN160410

Produktbeschreibung

Immunogen: Yeast-expressed, recombinant mouse GM-CSF.

Isotyp: IgG1, kappa chain

Klon: MP1-31G6

Beschreibung: Growth/development granulocyte/macrophage progenitors, differentiates myeloblasts/monoblasts, synergizes with Epo proliferation of erythroid/megakaryocytic progenitors T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells. The MP1-31G6 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The MP1-31G6 antibody can neutralize the bioactivity of natural or recombinant GM-CSF. Synonyms: Granulocyte/macrophage-colony stimulating factor, CSF-alpha, Pluripoietin-alpha, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA) Regulation: Synergistic with IL-1, IL-3, G-CSF, E21R competitive antagonist for receptor binding, stored in ECM with heparan sulfate proteoglycans Structure: Cytokine, 22 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA or ELISPOT Detection: The biotinylated MP1-31G6 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MP1-22E9 antibody (ABIN160402/ABIN160408) as the capture antibody. Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, and Neutralization. The LEAF(TM) purified MP1-22E9 antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse GM-CSF bioactivity in vivo and in vitro (ABIN160408). Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of GM-CSF recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-GM-CSF antibody (Biotin)

Publikationen

Publikationen: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Negrin, Greenberg: "Therapy of hematopoietic disorders with recombinant colony-stimulating factors." in: Advances in pharmacology (San Diego, Calif.), Vol. 23, pp. 263-96, 1992 (PubMed).

Demetri, Griffin: "Granulocyte colony-stimulating factor and its receptor." in: Blood, Vol. 78, Issue 11, pp. 2791-808, 1991 (PubMed).

Fan, OBrian, Ioannides et al.: "Granulocyte-macrophage colony-stimulating factor (GM-CSF) in the management of cancer." in: In vivo (Athens, Greece), Vol. 5, Issue 6, pp. 571-7, 1992 (PubMed).

Nozaki, Abrams, Pearce et al.: "Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes." in: The Journal of investigative dermatology, Vol. 97, Issue 1, pp. 10-4, 1991 (PubMed).

Suda, OGarra, MacNeil et al.: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, 1990 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).