IL-10 Antikörper (Biotin)

Antigen: IL-10

Klonalität: Monoklonal (JES5-16E3)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Biotin

Applikation: ELISA (Detektionsantikörper), ELISPOT (Detektionsantikörper), Intrazelluläre Durchflusszytometrie (ICFC)

Produktnummer: ABIN160376

Produktbeschreibung

Immunogen: E. coli-expressed, recombinant mouse IL-10

Isotyp: IgG2b, kappa chain

Klon: JES5-16E3

Beschreibung: Inhibits IFN-gamma, TNF-beta, IL-2 production by Th1 clones, inhibits macrophage-mediated IL-1, IL-6, TNF-alpha synthesis, suppresses delayed type hypersensitivity response, stimulates Th2 cell response, mast cell proliferation in combination with IL-3 or IL-4, B cell and thymocyte proliferation Activated CD8 T cells, Th0, Th2 subset of CD4 T cells, Ly-1 B cells, monocytes, macrophages, keratinocytes IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). The JES5-16E3 antibody can neutralize the bioactivity of natural or recombinant IL-10. Synonyms: Interleukin 10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF) Regulation: Downregulated by IL-4, IL-10 Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA or ELISPOT Detection: The biotinylated JES5-16E3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES5-2A5 antibody (ABIN160368/ABIN160370) as the capture antibody. ELISA or ELISPOT Capture: JES5-16E3 is useful as a capture antibody for sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated JES5-2A5 antibody (ABIN160372) as the detecting antibody. Flow Cytometry: The fluorochrome-labeled JES5-16E3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (ABIN160384). Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and paraformaldehyde-fixed, saponin-treated frozen tissue sections, In Vivo Capture, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For ELISA or ELISPOT detection, the antibody should be titrated between 0.5-2 µg/ml to determine optimal conditions. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For immunofluorescent staining, the suggested use of this reagent is < 0.25 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-10 antibody (Biotin)

Publikationen

Publikationen: Quesniaux: "Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview." in: Research in immunology, Vol. 143, Issue 4, pp. 385-400, 1992 (PubMed).

Howard, OGarra: "Biological properties of interleukin 10." in: Immunology today, Vol. 13, Issue 6, pp. 198-200, 1992 (PubMed).

Litton, Sander, Murphy et al.: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, 1994 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Khanna, Morelli, Zhong et al.: "Effects of liver-derived dendritic cell progenitors on Th1- and Th2-like cytokine responses in vitro and in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1346-54, 2000 (PubMed).

Simkin, Tao, Levy et al.: "IL-10 contributes to the inhibition of contact hypersensitivity in mice treated with photodynamic therapy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 5, pp. 2457-62, 2000 (PubMed).

Wang, Graeler, Goetzl: "Physiological sphingosine 1-phosphate requirement for optimal activity of mouse CD4+ regulatory T Cells." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 9, pp. 1043-5, 2004 (PubMed).

Brummel, Lenert: "Activation of marginal zone B cells from lupus mice with type A(D) CpG-oligodeoxynucleotides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 4, pp. 2429-34, 2005 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).