IL-10 Antikörper

Antigen: IL-10

Klonalität: Monoklonal (JES5-2A5)

Wirt: Ratte

Reaktivität: Maus

Applikation: ELISA (Fangantikörper), ELISPOT (Fangantikörper), ELISA (Detektionsantikörper), Neutralisierung (Neut), Western Blot (WB)

Produktnummer: ABIN160370

Produktbeschreibung

Immunogen: E. coli-expressed, recombinant mouse IL-10

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: JES5-2A5

Beschreibung: Inhibits IFN-gamma, TNF-beta, IL-2 production by TH1 clones, inhibits macrophage-mediated IL-1, IL-6, TNF-alpha synthesis, suppresses delayed type hypersensitivity response, stimulates TH2 cell response, mast cell proliferation in combination with IL-3 or IL-4, B cell and thymocyte proliferation Activated CD8 T cells, TH0, TH2 subset of CD4 T cells, Ly-1 B cells, monocytes, macrophages, keratinocytes IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody can neutralize the bioactivity of natural or recombinant IL-10. Synonyms: Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF) Regulation: Downregulated by IL-4, IL-10 Structure: Acid-labile cytokine, dimer, 17-21 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA or ELISPOT Capture: The purified JES5-2A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES5-16E3 antibody (ABIN160376) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. ELISA or ELISPOT Detection: The biotinylated JES5-2A5 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES5-16E3 antibody (ABIN160374/ABIN160384) as the capture antibody. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (ABIN160370). Additional reported applications (for the relevant formats) include: Western blotting, In Vivo Capture. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For ELISA and ELISPOT capture applications, a concentration range of 4-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. * For ELISA/ELISPOT capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4, q.s. to 1.0 L) as coating buffer. Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-10 antibody

Publikationen

Publikationen: Quesniaux: "Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview." in: Research in immunology, Vol. 143, Issue 4, pp. 385-400, 1992 (PubMed).

Howard, OGarra: "Biological properties of interleukin 10." in: Immunology today, Vol. 13, Issue 6, pp. 198-200, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995 (PubMed).

Sarawar, Sangster, Coffman et al.: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, 1994 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Hara, Kingsley, Niimi et al.: "IL-10 is required for regulatory T cells to mediate tolerance to alloantigens in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 3789-96, 2001 (PubMed).

Brummel, Lenert: "Activation of marginal zone B cells from lupus mice with type A(D) CpG-oligodeoxynucleotides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 4, pp. 2429-34, 2005 (PubMed).

Riemann, Endres, Liptay et al.: "The IkappaB protein Bcl-3 negatively regulates transcription of the IL-10 gene in macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 6, pp. 3560-8, 2005 (PubMed).