IL-2 Antikörper (Biotin)

Antigen: IL-2

Klonalität: Monoklonal (JES6-5H4)

Wirt: Ratte

Reaktivität: Maus

Konjugat: Biotin

Applikation: ELISA (Detektionsantikörper), ELISPOT (Detektionsantikörper), Intrazelluläre Durchflusszytometrie (ICFC)

Produktnummer: ABIN160305

Produktbeschreibung

Immunogen: E. coli-expressed, recombinant mouse IL-2

Isotyp: IgG2b, kappa chain

Klon: JES6-5H4

Beschreibung: Proliferation of T lymphocytes, B cells, anti-inflammatory, hematopoiesis, tumor surveillance T cells IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes. The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2). This is a neutralizing antibody. Synonyms: Interleukin-2, T cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF) Regulation: Upregulated by NFAT, downregulated by TCF-8, CIF (colostrum inhibitory factor) Structure: Cytokine, 15-30 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA Detection or ELISPOT Detection: The biotinylated JES6-5H4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES6-1A12 antibody (ABIN160299/ABIN160301) as capture antibody and recombinant mouse IL-2 (ABIN160656) as the standard. Flow Cytometry: The fluorochrome-labeled JES6-5H4 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-2 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of mouse IL-2 bioactivity in vivo and in vitro (ABIN160313). Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, In Vivo Capture, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-2 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-2 antibody (Biotin)

Publikationen

Publikationen: Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

Mo, Sarawar, Doherty: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, 1995 (PubMed).

Nistico: "Communications among central nervous system, neuroendocrine and immune systems: interleukin-2." in: Progress in neurobiology, Vol. 40, Issue 4, pp. 463-75, 1993 (PubMed).

Sander, Hoeiduen, Andersson et al.: "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." in: Journal of immunological methods, Vol. 166, Issue 2, pp. 201-14, 1994 (PubMed).

Waldmann, Goldman, Top et al.: "The interleukin-2 receptor: a target for immunotherapy." in: Annals of the New York Academy of Sciences, Vol. 685, pp. 603-10, 1993 (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, 1993 (PubMed).

Karulin, Hesse, Tary-Lehmann et al.: "Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1862-72, 2000 (PubMed).

Kang, Allen: "Priming in the presence of IL-10 results in direct enhancement of CD8+ T cell primary responses and inhibition of secondary responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 9, pp. 5382-9, 2005 (PubMed).

Ko, Ko, Chang et al.: "alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 5, pp. 3309-17, 2005 (PubMed).

Lawson, Manenkova, Ahamed et al.: "Inhibition of transmethylation down-regulates CD4 T cell activation and curtails development of autoimmunity in a model system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 5366-74, 2007 (PubMed).