IL-13 Antikörper

Antigen: IL-13

Klonalität: Monoklonal (JES10-5A2)

Wirt: Ratte

Reaktivität: Human

Applikation: ELISA (Fangantikörper), ELISPOT (Fangantikörper), Neutralisierung (Neut), Intrazelluläre Durchflusszytometrie (ICFC), Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN160200

Produktbeschreibung

Immunogen: COS-expressed, recombinant human IL-13

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: JES10-5A2

Beschreibung: Suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1, differentiation of B cells, monocytes Activated T cells, mast cells, NK cells IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13. Synonyms: Interleukin-13, NC30 Regulation: IL-13Ralpha2 possible antagonist Structure: Cytokine, 17 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA or ELISPOT Capture: The purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (ABIN160201) as the detecting antibody. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled JES10-5A2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-13 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of human IL-13 bioactivity (ABIN160200). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. Each lot of this antibody is quality control tested by ELISA analysis. For ELISA applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-13 antibody

Publikationen

Publikationen: Obiri, Debinski, Leonard et al.: "Receptor for interleukin 13. Interaction with interleukin 4 by a mechanism that does not involve the common gamma chain shared by receptors for interleukins 2, 4, 7, 9, and 15." in: The Journal of biological chemistry, Vol. 270, Issue 15, pp. 8797-804, 1995 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Skansuen-Saphir, Andersson, Bjoerk et al.: "Lymphokine production induced by streptococcal pyrogenic exotoxin-A is selectively down-regulated by pooled human IgG." in: European journal of immunology, Vol. 24, Issue 4, pp. 916-22, 1994 (PubMed).

Hilton, Zhang, Metcalf et al.: "Cloning and characterization of a binding subunit of the interleukin 13 receptor that is also a component of the interleukin 4 receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 1, pp. 497-501, 1996 (PubMed).

Ochensberger, Daepp, Rihs et al.: "Human blood basophils produce interleukin-13 in response to IgE-receptor-dependent and -independent activation." in: Blood, Vol. 88, Issue 8, pp. 3028-37, 1996 (PubMed).

Knoerr, Amrehn, Seeberger et al.: "Expression of costimulatory molecules in low-grade mucosa-associated lymphoid tissue-type lymphomas in vivo." in: The American journal of pathology, Vol. 155, Issue 6, pp. 2019-27, 1999 (PubMed).

Schaerli, Willimann, Lang et al.: "CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1553-62, 2000 (PubMed).

Gelfand, Dakhama: "CD8+ T lymphocytes and leukotriene B4: novel interactions in the persistence and progression of asthma." in: The Journal of allergy and clinical immunology, Vol. 117, Issue 3, pp. 577-82, 2006 (PubMed).