IL-10 Antikörper (Biotin)

Antigen: IL-10

Klonalität: Monoklonal (JES3-12G8)

Wirt: Ratte

Reaktivität: Human

Konjugat: Biotin

Applikation: ELISA (Detektionsantikörper), ELISPOT (Detektionsantikörper)

Produktnummer: ABIN160171

Produktbeschreibung

Immunogen: COS - expressed, recombinant human IL-10

Isotyp: IgG2a, kappa chain

Klon: JES3-12G8

Beschreibung: Inhibit IFN-gamma, TNF-beta, IL-2 production by TH1 clones, inhibits macrophage-mediated IL-1, IL-6, TNF-alpha synthesis, suppress delayed type hypersensitivity response, stimulate TH2 cell response, mast cell proliferation in combination with IL-3 or IL-4, B cell and thymocyte proliferation Activated CD8 and CD4 T cells, activated monocytes, mast cells, Ly-1 B (mouse) IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody can neutralize the bioactivity of natural or recombinant IL-10. Synonyms: Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF) Regulation: Production inhibited by IL-4, IL-10 Structure: Acid-labile cytokine, dimer, 35-40 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA Detection or ELISPOT Detection: The biotinylated JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified JES3-3-9D7 antibody (ABIN160157/ABIN160162) or purified JES3-19F1 antibody (ABIN160487/ABIN160495) as capture antibody and recombinant human IL-10 (ABIN160642) as the standard. Biotin-F(ab') (ABIN160172) is recommended for ELISA analysis of human serum and plasma samples, to prevent non-specific binding of interfering substances such as rheumatoid factor and heterophilic antibodies. Each lot of this antibody is quality control tested by ELISA assay. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, neutralization, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-10 antibody (Biotin)

Publikationen

Publikationen: Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).

Quesniaux: "Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview." in: Research in immunology, Vol. 143, Issue 4, pp. 385-400, 1992 (PubMed).

Howard, OGarra: "Biological properties of interleukin 10." in: Immunology today, Vol. 13, Issue 6, pp. 198-200, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

de Waal Malefyt, Yssel, Roncarolo et al.: "Interleukin-10." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 314-20, 1992 (PubMed).

Gotlieb, Abrams, Watson et al.: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, 1992 (PubMed).

Andersson, Abrams, Bjoerk et al.: "Concomitant in vivo production of 19 different cytokines in human tonsils." in: Immunology, Vol. 83, Issue 1, pp. 16-24, 1995 (PubMed).

Burdin, Pueronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed).

Tazi, Moreau, Bergeron et al.: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999 (PubMed).