IL-10 Antikörper

Antigen: IL-10

Klonalität: Monoklonal (JES3-9D7)

Wirt: Ratte

Reaktivität: Human

Applikation: ELISA (Fangantikörper), ELISPOT (Fangantikörper), Neutralisierung (Neut), Intrazelluläre Durchflusszytometrie (ICFC), Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN160162

Produktbeschreibung

Immunogen: COS - expressed, recombinant human IL-10

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: JES3-9D7

Beschreibung: Inhibit IFN-gamma, TNF-beta, IL-2 production by TH1 clones, inhibits macrophage-mediated IL-1, IL-6, TNF-alpha synthesis, suppress delayed type hypersensitivity response, stimulate TH2 cell response, mast cell proliferation in combination with IL-3 or IL-4, B cell and thymocyte proliferation Activated CD8 and CD4 T cells, activated monocytes, mast cells, Ly-1 B (mouse) IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10. Synonyms: Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF) Regulation: Production inhibited by IL-4, IL-10 Structure: Acid-labile cytokine, dimer, 35-40 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA Capture or ELISPOT Capture: The purified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (ABIN160171) as the detecting antibody and recombinant human IL-10 (ABIN160642) as the standard. The LEAF(TM) purified antibody is suggested for ELISPOT capture. Flow Cytometry: The fluorochrome-labeled JES3-9D7 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.com and click on the support section. Neutralization: The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for neutralization of human IL-10 bioactivity (ABIN160162). Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry. No. to) are specially developed and recommended. Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 4-8 µg/ml is recommended. For ELISA capture applications, a concentration range of 2-6 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-IL-10 antibody

Publikationen

Publikationen: Yssel, De Waal Malefyt, Roncarolo et al.: "IL-10 is produced by subsets of human CD4+ T cell clones and peripheral blood T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2378-84, 1992 (PubMed).

Quesniaux: "Interleukins 9, 10, 11 and 12 and kit ligand: a brief overview." in: Research in immunology, Vol. 143, Issue 4, pp. 385-400, 1992 (PubMed).

Howard, OGarra: "Biological properties of interleukin 10." in: Immunology today, Vol. 13, Issue 6, pp. 198-200, 1992 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, pp. Mai 24, 1992 (PubMed).

de Waal Malefyt, Yssel, Roncarolo et al.: "Interleukin-10." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 314-20, 1992 (PubMed).

Gotlieb, Abrams, Watson et al.: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, 1992 (PubMed).

Burdin, Pueronne, Banchereau et al.: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, 1993 (PubMed).

Akdis, Blesken, Akdis et al.: "Role of interleukin 10 in specific immunotherapy." in: The Journal of clinical investigation, Vol. 102, Issue 1, pp. 98-106, 1998 (PubMed).

Jason, Archibald, McDonald et al.: "Immune determinants of organism and outcome in febrile hospitalized Thai patients with bloodstream infections." in: Clinical and diagnostic laboratory immunology, Vol. 6, Issue 1, pp. 73-8, 1999 (PubMed).

Schaerli, Willimann, Lang et al.: "CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function." in: The Journal of experimental medicine, Vol. 192, Issue 11, pp. 1553-62, 2000 (PubMed).