V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) Antikörper

Antigen: V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyme: ERBB3

Klonalität: Monoklonal (1B4C3)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN159487

Produktbeschreibung

Weitere Bezeichnung: erbB3/HER-3

Immunogen: NIH-3T3 cells transfected with human erbB3/HER3

Format: Purified

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: 1B4C3

Beschreibung: The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors, expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5), Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3/HER3. Distribution: Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors, expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer Function: Induces extensive signaling cascade that can regulate growth and possibly differentiation Modifications: Extensively phosphorylated T873 and S1123 (by cdk5), Y1199, Y1262, Y1289, Y1328 Synonyms: Oncogene erbB3, tyrosine kinase cell surface receptor HER3, C-erbB3 Structure: Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD

Anwendungen

Applikationshinweise: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) antibody

Publikationen

Publikationen: Kraus, Issing, Miki et al.: "Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 23, pp. 9193-7, 1990 (PubMed).

Chan, Antoniucci, Fok et al.: "Heregulin activation of extracellular acidification in mammary carcinoma cells is associated with expression of HER2 and HER3." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22608-13, 1995 (PubMed).

Carraway, Sliwkowski, Akita et al.: "The erbB3 gene product is a receptor for heregulin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14303-6, 1994 (PubMed).