Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR) Antikörper

Antigen: Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR)

Synonyme: CD18, TNFCR, D12S370, TNFR-RP, TNFRSF3, TNFR2-RP, LT-BETA-R, TNF-R-III

Klonalität: Monoklonal (31G4D8)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Immunpräzipitation (IP), Apoptose-Induktion (AI)

Produktnummer: ABIN159279

Produktbeschreibung

Weitere Bezeichnung: Lymphotoxin beta Receptor (LT-betaR)

Immunogen: Human LT-betaR Fc protein

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2b, kappa chain  (Passende Sekundärantikörper)

Klon: 31G4D8

Beschreibung: LT-betaR, also known as lymphotoxin beta receptor and tumor necrosis factor receptor superfamily member 3 is a 47 kD transmembrane protein containing 3 TNF receptor domains. The LT-betaR is a receptor for the heterotrimeric lymphotoxin complex (composed of LTalpha and LTbeta as well as LIGHT. LT-betaR is highly expressed in the lung, liver, and kidney and moderately expressed expressed in the heart and testes. LT-betaR is weakly expressed in the brain, thymus, spleen, and lymph nodes. The cytoplasmic tail of LT-betaR interacts with TRAF3, TRAF4, and TRAF5 as well as the hepatitis C virus core protein. Ligand binding to LT-betaR has been shown to induce apoptosis (via TRAF3 and TRAF5) and play a role in the development of lymphoid organs. The 31G4D8 antibody has been shown to be useful for flow cytometry, Western blot, immunoprepicitation and and ELISA (detection antibody). In addition, the 31G4D8 antibody is an agonistic antibody for the LT-betaR capable of inducing apoptosis (with IFN-gamma treatment). Distribution: Highly expressed in lung, lever, and kidney. Moderate expression in heart and testes, weak expression in brain, thymus, spleen, and lymph nodes. Function: Promotes apoptosis via TRAF3 and TRAF5, plays a role in the development of lymphoid organs Synonyms: Lymphotoxin beta receptor, Tumor necrosis factor receptor superfamily member 3 Structure: Member of the TNF receptor superfamily, transmembrane receptor containing 3 TNF receptor domains, predicted molecular weight approximately 47 kD

Spezifität: Receptor for heterotrimeric lymphotoxin complex (composed of LTalpha and LTbeta as well as LIGHT. Also interacts with TRAF3, TRAF4, and TRAF5 as well as hepatitis C virus core protein.

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, ELISA detection, induction of apoptosis. The biotinylated 31G4D8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with the purified 36G9G3 antibody (/) as capture antibody. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN159278/ABIN159279). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 2.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Toxine, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Lymphotoxin beta Receptor (TNFR Superfamily, Member 3) (LTBR) antibody

Publikationen

Publikationen: Crowe, VanArsdale, Walter et al.: "A lymphotoxin-beta-specific receptor." in: Science (New York, N.Y.), Vol. 264, Issue 5159, pp. 707-10, 1994 (PubMed).

Nakano, Oshima, Chung et al.: "TRAF5, an activator of NF-kappaB and putative signal transducer for the lymphotoxin-beta receptor." in: The Journal of biological chemistry, Vol. 271, Issue 25, pp. 14661-4, 1996 (PubMed).

Wu, Wang, Han et al.: "The cytoplasmic domain of the lymphotoxin-beta receptor mediates cell death in HeLa cells." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 11868-73, 1999 (PubMed).

Rooney, Butrovich, Glass et al.: "The lymphotoxin-beta receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14307-15, 2000 (PubMed).

Chen, Hwang, Chou et al.: "The role of apoptosis signal-regulating kinase 1 in lymphotoxin-beta receptor-mediated cell death." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16073-81, 2003 (PubMed).