Mannose Receptor, C Type 1 (MRC1) Antikörper

Antigen: Mannose Receptor, C Type 1 (MRC1)

Synonyme: MMR, CD206, CLEC13D, MR, AW259686, mrc1, MGC86525, MRC1L1, MRC1

Klonalität: Monoklonal (15-Feb)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN159212

Produktbeschreibung

Weitere Bezeichnung: CD206 (MMR)

Immunogen: purified human mannose receptor

Format: Purified

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: 15-Feb

Beschreibung: Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein, also know as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, and plays an important role in host defense and provides a link between innate and adaptive immunity. The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Distribution: Macrophages, dendritic cells, hepatic and lymphatic endothelial cells Function: Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation Synonyms: MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1 Structure: Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD.

Anwendungen

Applikationshinweise: Additional reported applications of this antibody (for the relevant formats) include: Westerb blotting, blocking of ligand binding, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN159223). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or per 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Wileman, Lennartz, Stahl: "Identification of the macrophage mannose receptor as a 175-kDa membrane protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 8, pp. 2501-5, 1986 (PubMed).

Noorman, Braat, Barrett-Bergshoeff et al.: "Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages." in: Journal of leukocyte biology, Vol. 61, Issue 1, pp. 63-72, 1997 (PubMed).

Barrett-Bergshoeff, Noorman, Bos et al.: "Monoclonal antibodies against the human mannose receptor that inhibit the binding of tissue-type plasminogen activator." in: Thrombosis and haemostasis, Vol. 77, Issue 4, pp. 718-24, 1997 (PubMed).

Apostolopoulos, McKenzie: "Role of the mannose receptor in the immune response." in: Current molecular medicine, Vol. 1, Issue 4, pp. 469-74, 2002 (PubMed).

Le Cabec, Emorine, Toesca et al.: "The human macrophage mannose receptor is not a professional phagocytic receptor." in: Journal of leukocyte biology, Vol. 77, Issue 6, pp. 934-43, 2005 (PubMed).

Desguerre, Mayer, Leturcq et al.: "Endomysial fibrosis in Duchenne muscular dystrophy: a marker of poor outcome associated with macrophage alternative activation." in: Journal of neuropathology and experimental neurology, Vol. 68, Issue 7, pp. 762-73, 2009 (PubMed).