zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) Antikörper (PE)

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyme: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Klonalität: Monoklonal (1E7-2)

Wirt: Maus

Reaktivität: Human, Maus

Konjugat: PE

Applikation: Intrazelluläre Durchflusszytometrie (ICFC)

Produktnummer: ABIN158749

Produktbeschreibung

Weitere Bezeichnung: ZAP-70

Immunogen: GST-fusion protein corresponding to residues 282-307 of human ZAP-70

Isotyp: IgG1, IgG1 kappa

Klon: 1E7-2

Beschreibung: ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation. Distribution: T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL) Function: Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect Modifications: Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity) Synonyms: Zeta chain associated protein kinase, SYK related tyrosine kinase Structure: Protein tyrosine kinase, contains two SH2 domains, 70 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per 1 million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: Signaltransduktion, Tags/Labels, Immunologie, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (PE)

Publikationen

Publikationen: Chan, Iwashima, Turck et al.: "ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain." in: Cell, Vol. 71, Issue 4, pp. 649-62, 1992 (PubMed).

Arpaia, Shahar, Dadi et al.: "Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase." in: Cell, Vol. 76, Issue 5, pp. 947-58, 1994 (PubMed).

Chan, Kadlecek, Elder et al.: "ZAP-70 deficiency in an autosomal recessive form of severe combined immunodeficiency." in: Science (New York, N.Y.), Vol. 264, Issue 5165, pp. 1599-601, 1994 (PubMed).

Qian, Lev, van Oers et al.: "Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling." in: The Journal of experimental medicine, Vol. 185, Issue 7, pp. 1253-9, 1997 (PubMed).

Crespo, Bosch, Villamor et al.: "ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia." in: The New England journal of medicine, Vol. 348, Issue 18, pp. 1764-75, 2003 (PubMed).