CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) Antikörper

Antigen: CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40)

Synonyme: p50, Bp50, CDW40, MGC9013, TNFRSF5, IGM, GP39, IMD3, TRAP, HIGM1, T-BAM, Tnfrsf5, AI326936, CD40

Klonalität: Monoklonal (HB14)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Kostimulation

Produktnummer: ABIN158723

Produktbeschreibung

Weitere Bezeichnung: CD40

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: HB14

Beschreibung: CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis. Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis Synonyms: BP50, TNFRSF5 Structure: TNFR superfamily, type I glycoprotein, 48 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, and partial inhibition of CD40 binding to CD40L and rescue B cell from apoptosis. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158723). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes stained with LEAF(TM) purified HB14, followed by anti-mouse IgGs FITC

Publikationen

Publikationen: Banchereau, Bazan, Blanchard et al.: "The CD40 antigen and its ligand." in: Annual review of immunology, Vol. 12, pp. 881-922, 1994 (PubMed).

Foy, Aruffo, Bajorath et al.: "Immune regulation by CD40 and its ligand GP39." in: Annual review of immunology, Vol. 14, pp. 591-617, 1996 (PubMed).

Pound, Challa, Holder et al.: "Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells." in: International immunology, Vol. 11, Issue 1, pp. 11-20, 1999 (PubMed).

Armengol, Juan, Lucas-Martin et al.: "Thyroid autoimmune disease: demonstration of thyroid antigen-specific B cells and recombination-activating gene expression in chemokine-containing active intrathyroidal germinal centers." in: The American journal of pathology, Vol. 159, Issue 3, pp. 861-73, 2001 (PubMed).

Cavanagh, Boyce, Smith et al.: "Rheumatoid arthritis synovium contains plasmacytoid dendritic cells." in: Arthritis research & therapy, Vol. 7, Issue 2, pp. R230-40, 2005 (PubMed).