CD180 Molecule (CD180) Antikörper

Antigen: CD180 Molecule (CD180)

Synonyme: LY64, Ly78, RP105, MGC126233, MGC126234, Lrrp, Ly64, NJ-1, 14/A10, 114/A10, AI159736, CD180, Gir, RP39, RP82, Gpr72, AW045253, F630107B15

Klonalität: Monoklonal (MHR73-11)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Intrazelluläre Durchflusszytometrie (ICFC), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN158708

Produktbeschreibung

Weitere Bezeichnung: CD180 (RP105)

Immunogen: Human RP105 complexed with mouse MD-1

Format: Purified

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: MHR73-11

Beschreibung: CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis. Distribution: B cells, monocytes/macrophages, and dendritic cells Function: Signaling protein involved in innate immunity Synonyms: CD180, Radio-protective 105-kD protein, RP105 Structure: Member of the toll-like receptor family, 105 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: intracellular staining, immunoprecipitation, immunohistochemistry of acetone-fixed frozen tissue sections, B cell activation, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1). The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158713). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Buffer: This antibody was purified by affinity chromatography.

Lagerung: The antibody solution should be stored undiluted at 4°C.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes stained with purified MHR73-11, followed by anti-mouse IgG FITC

Publikationen

Publikationen: Miura, Miyake, Yamashita et al.: "Molecular cloning of a human RP105 homologue and chromosomal localization of the mouse and human RP105 genes (Ly64 and LY64)." in: Genomics, Vol. 38, Issue 3, pp. 299-304, 1997 (PubMed).

Miura, Shimazu, Miyake et al.: "RP105 is associated with MD-1 and transmits an activation signal in human B cells." in: Blood, Vol. 92, Issue 8, pp. 2815-22, 1998 (PubMed).