Major Histocompatibility Complex, Class I, A (HLA-A) Antikörper

Antigen: Major Histocompatibility Complex, Class I, A (HLA-A)

Synonyme: HLAA, FLJ26655

Klonalität: Monoklonal (W6-32)

Wirt: Maus

Reaktivität: Human, Rhesusaffen, Cynomolgus, Pavian, Katze, Rind (Kuh)

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Blockierende Antikörper (Inhibition), Aktivierung (Act)

Produktnummer: ABIN158631

Produktbeschreibung

Weitere Bezeichnung: HLA-A

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: W6-32

Beschreibung: The W6/32 monoclonal antibody reacts with a non-polymorphic epitope of human major histocompatibility complex (MHC) class I antigens, HLA-A, B, C. MHC class I antigens associated with beta2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 T cells. They play an important role in cell-mediated immune responses and tumor surveillance. W6/32 cross reacts with rhesus, cynomogus, baboon, feline, and bovine. Distribution: All nucleated cells Function: Antigen presentation Synonyms: Major Histocompatibility Class I, MHC class I Structure: Ig superfamily

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitaton, Western blotting (non-reducing), Immunohistochemical staining of acetone-fixed frozen tissue sections, blocking, inhibition of NK cell-mediated lysis, and activation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158631). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 2.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Haupthistokompatibilitätskomplex (MHC)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Major Histocompatibility Complex, Class I, A (HLA-A) antibody

Publikationen

Publikationen: Geppert, Wacholtz, Patel et al.: "Activation of human T cell clones and Jurkat cells by cross-linking class I MHC molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 11, pp. 3763-72, 1989 (PubMed).

Darrow, Slingluff, Seigler: "The role of HLA class I antigens in recognition of melanoma cells by tumor-specific cytotoxic T lymphocytes. Evidence for shared tumor antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 9, pp. 3329-35, 1989 (PubMed).

Turco, de Felice, Corbo et al.: "Enhancing effect of anti-HLA class I monoclonal antibodies on T cell proliferation induced via CD2 molecule." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 7, pp. 2275-81, 1988 (PubMed).

Stern, Beresford, Friedman et al.: "Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 4, pp. 1088-91, 1987 (PubMed).

Barbatis, Woods, Morton et al.: "Immunohistochemical analysis of HLA (A, B, C) antigens in liver disease using a monoclonal antibody." in: Gut, Vol. 22, Issue 12, pp. 985-91, 1982 (PubMed).

Fayen, Huang, Ferrone et al.: "Negative signaling by anti-HLA class I antibodies is dependent upon two triggering events." in: International immunology, Vol. 10, Issue 9, pp. 1347-58, 1998 (PubMed).

Tran, Ivanyi, Hilgert et al.: "The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex." in: Immunogenetics, Vol. 53, Issue 6, pp. 440-6, 2001 (PubMed).

Ayyoub, Taub, Keohan et al.: "The frequent expression of cancer/testis antigens provides opportunities for immunotherapeutic targeting of sarcoma." in: Cancer immunity : a journal of the Academy of Cancer Immunology, Vol. 4, pp. 7, 2004 (PubMed).

Wooden, Kalb, Cotter et al.: "Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1383-7, 2005 (PubMed).

Nagano, Yamashita, Hamada et al.: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, 2007 (PubMed).