PCNA Antikörper (Biotin)

Antigen: PCNA

Klonalität: Monoklonal (PC10)

Wirt: Maus

Reaktivität: Human, Maus, Diverse Spezies

Konjugat: Biotin

Applikation: Intrazelluläre Durchflusszytometrie (ICFC), Immunfluoreszenz (IF)

Produktnummer: ABIN158385

Produktbeschreibung

Immunogen: Recombinant rat PCNA

Isotyp: IgG2a, kappa chain

Klon: PC10

Beschreibung: The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase delta auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase delta processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody. Distribution: Nuclear, all proliferating cells Function: RAD6-dependent DNA repair pathway, increases DNA polymerase delta processibility during elongation of the leading strand Modifications: Ubiquitination, Sumoylation Synonyms: Proliferating Cell Nuclear Antigen, DNA Polymerase delta Auxiliary Protein Structure: DNA-polymerase sliding clamp, trimeric ring, 36 kD

Spezifität: PCNA, DNA polymerase delta, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5.

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry, immunofluorescence microscopy, and Western blotting. Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C. Do not freeze.

Forschungsgebiet: Proliferationsmarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Wilkins, Harris, Waseem et al.: "A study of cell proliferation in formalin-fixed, wax-embedded bone marrow trephine biopsies using the monoclonal antibody PC10, reactive with proliferating cell nuclear antigen (PCNA)." in: The Journal of pathology, Vol. 166, Issue 1, pp. 45-52, 1992 (PubMed).

Landberg, Roos: "Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis." in: Cancer research, Vol. 51, Issue 17, pp. 4570-4, 1991 (PubMed).

Woods, Hall, Shepherd et al.: "The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis." in: Histopathology, Vol. 19, Issue 1, pp. 21-7, 1991 (PubMed).

Yu, Hall, Fletcher et al.: "Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA)." in: Histopathology, Vol. 19, Issue 1, pp. 29-33, 1991 (PubMed).

Waseem, Lane: "Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form." in: Journal of cell science, Vol. 96 ( Pt 1), pp. 121-9, 1990 (PubMed).

Landberg, Tan, Roos: "Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle." in: Experimental cell research, Vol. 187, Issue 1, pp. 111-8, 1990 (PubMed).

Hall, Levison, Woods et al.: "Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms." in: The Journal of pathology, Vol. 162, Issue 4, pp. 285-94, 1991 (PubMed).

Garcia, Coltrera, Gown: "Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis." in: The American journal of pathology, Vol. 134, Issue 4, pp. 733-9, 1989 (PubMed).

Travali, Ku, Rizzo et al.: "Structure of the human gene for the proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 264, Issue 13, pp. 7466-72, 1989 (PubMed).

Ogata, Ogata, Nakamura et al.: "Purification and N-terminal amino acid sequence of proliferating cell nuclear antigen (PCNA)/cyclin and development of ELISA for anti-PCNA antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 4, pp. 2623-7, 1985 (PubMed).

Ying, Yang: "[Angiotensin II and the adrenal gland]" in: Sheng li ke xue jin zhan [Progress in physiology], Vol. 27, Issue 1, pp. 70-2, 1996 (PubMed).

Galkowska, Waldemar, Wojewodzka: "Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes." in: Veterinary immunology and immunopathology, Vol. 53, Issue 3-4, pp. 329-34, 1997 (PubMed).

Hoege, Pfander, Moldovan et al.: "RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMO." in: Nature, Vol. 419, Issue 6903, pp. 135-41, 2002 (PubMed).

Yue, Na, Feng et al.: "Expression of p57kip2, Rb protein and PCNA and their relationships with clinicopathology in human pancreatic cancer." in: World journal of gastroenterology : WJG, Vol. 9, Issue 2, pp. 377-80, 2003 (PubMed).

Shan, Xu, Zhuo et al.: "Induction of p53-dependent activation of the human proliferating cell nuclear antigen gene in chromatin by ionizing radiation." in: The Journal of biological chemistry, Vol. 278, Issue 45, pp. 44009-17, 2003 (PubMed).

Di Fulvio, Lehman, Lin et al.: "The elucidation of novel SH2 binding sites on PLD2." in: Oncogene, Vol. 25, Issue 21, pp. 3032-40, 2006 (PubMed).

Eswarakumar, Schlessinger: "Skeletal overgrowth is mediated by deficiency in a specific isoform of fibroblast growth factor receptor 3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 10, pp. 3937-42, 2007 (PubMed).