Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) Antikörper

Antigen: Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1)

Synonyme: SLAM, CD150, CDw150, SLAMF1, RGD1560634

Klonalität: Monoklonal (A12 (7D4))

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Kostimulation, Immunpräzipitation (IP)

Produktnummer: ABIN158280

Produktbeschreibung

Weitere Bezeichnung: CD150 (SLAM)

Immunogen: Activated human PBMC

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: A12 (7D4)

Beschreibung: CD150 is a 70-95 kD type I transmembrane glycoprotein also known as SLAM or IPO-3. It is a member of the Ig superfamily. It is expressed on a subset of T cells, B cells, dendritic cells, and endothelial cells. The expression of CD150 is upregulated upon activation. CD150 binds to itself as the ligand to be involved in B cell costimulation, proliferation, immunoglobulin production, and signal transduction. Distribution: T subset (upregulated after activation), B cells, dendritic cells, endothelial cells Function: B cell costimulation, proliferation and Ig production Synonyms: SLAM, Signaling Lymphocyte Activation Molecule, IPO-3 Structure: Ig superfamily, type I transmembrane glycoprotein, 70-95 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation, and costimulation of IFN-gamma production and T cell proliferation. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158280). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1) antibody

Publikationen

Publikationen: Pinchouk, Sidorenko, Gluzman et al.: "Monoclonal antibodies IPO-3 and IPO-10 against human B cell differentiation antigens." in: Anticancer research, Vol. 8, Issue 6, pp. 1377-80, 1989 (PubMed).

Cocks, Chang, Carballido et al.: "A novel receptor involved in T-cell activation." in: Nature, Vol. 376, Issue 6537, pp. 260-3, 1995 (PubMed).

Polacino, Pinchuk, Sidorenko et al.: "Immunodeficiency virus cDNA synthesis in resting T lymphocytes is regulated by T cell activation signals and dendritic cells." in: Journal of medical primatology, Vol. 25, Issue 3, pp. 201-9, 1997 (PubMed).

Punnonen, Cocks, Carballido et al.: "Soluble and membrane-bound forms of signaling lymphocytic activation molecule (SLAM) induce proliferation and Ig synthesis by activated human B lymphocytes." in: The Journal of experimental medicine, Vol. 185, Issue 6, pp. 993-1004, 1997 (PubMed).

Aversa, Chang, Carballido et al.: "Engagement of the signaling lymphocytic activation molecule (SLAM) on activated T cells results in IL-2-independent, cyclosporin A-sensitive T cell proliferation and IFN-gamma production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 9, pp. 4036-44, 1997 (PubMed).

Sayós, Martín, Chen et al.: "Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP." in: Blood, Vol. 97, Issue 12, pp. 3867-74, 2001 (PubMed).

Garcia, Quiroga, Ochoa et al.: "Signaling lymphocytic activation molecule expression and regulation in human intracellular infection correlate with Th1 cytokine patterns." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5719-24, 2001 (PubMed).

Vincent, Tigaud, Schneider et al.: "Restriction of measles virus RNA synthesis by a mouse host cell line: trans-complementation by polymerase components or a human cellular factor(s)." in: Journal of virology, Vol. 76, Issue 12, pp. 6121-30, 2002 (PubMed).