CD95 Antikörper

Antigen: CD95

Klonalität: Monoklonal (EOS9-1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Apoptose-Induktion (AI)

Produktnummer: ABIN158224

Produktbeschreibung

Weitere Bezeichnung: CD95 (FAS)

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgM, kappa chain  (Passende Sekundärantikörper)

Klon: EOS9-1

Beschreibung: CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis, and has been shown to play a role in the maintenance of peripheral tolerance. The EOS9.1 antibody is useful for inducing apoptosis of Fas-positive cells. Distribution: T and B cells, monocytes, neutrophils, expression level upregulated on activated lymphocytes Function: Mediates apoptosis Synonyms: Fas, APO-1, TNFRSF6 Structure: TNFR superfamily, type I transmembrane protein, 45 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN158224). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 2.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4°C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Nagata, Golstein: "The Fas death factor." in: Science (New York, N.Y.), Vol. 267, Issue 5203, pp. 1449-56, 1995 (PubMed).

Krammer, Dhein, Walczak et al.: "The role of APO-1-mediated apoptosis in the immune system." in: Immunological reviews, Vol. 142, pp. 175-91, 1995 (PubMed).