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GAP43 Antikörper (Growth Associated Protein 43)

Details für Produkt anti-GAP43 Antikörper Nr. ABIN1580421, Anbieter: Anmelden zum Anzeigen
Antigen
  • b-50
  • B-50
  • Basp2
  • cb310
  • gap
  • GAP-43
  • gap-43
  • gap43
  • gap43a
  • gap43b
  • pp46
  • PP46
Reaktivität
Ratte (Rattus), Maus
172
155
124
33
13
11
10
9
8
7
5
5
3
2
2
1
1
1
Wirt
Maus
182
40
9
6
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GAP43 Antikörper ist unkonjugiert
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
145
71
66
48
44
42
34
25
22
4
3
1
Hersteller
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Klon 1E3
Isotyp IgG1
Reinigung affinity purified antibody
Andere Bezeichnung GAP43 (GAP43 Antibody Abstract)
Hintergrund GAP43 is very abundant nervous system protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14KDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomolously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2 and tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications. The HGNC name for this protein is GAP43.
Forschungsgebiet Neurology
Applikationshinweise The antibody solution can be used at dilutions of at least 1:1,000 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:10,000 or lower.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer The antibody solution is affinity purified from tissue culture supernatant and is at concentration of 1mg/ml in phosphate buffered saline preparation containing 10 mM sodium azide preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at 4°C short term or -20°C long term.
Bilder des Herstellers
Western Blotting (WB) image for anti-GAP43 Antikörper (Growth Associated Protein 43) (ABIN1580421) Western blot of whole rat spinal cord lysates probed with ABIN1580421 antibody to GAP...
Immunofluorescence (IF) image for anti-GAP43 Antikörper (Growth Associated Protein 43) (ABIN1580421) Mixed neuronal cultures stained with ABIN1580421 (green), RPCA-MAP2, a rabbit antibod...
Allgemeine Veröffentlichungen Skene, Willard: "Changes in axonally transported proteins during axon regeneration in toad retinal ganglion cells." in: The Journal of cell biology, Vol. 89, Issue 1, pp. 86-95, 1981