CD33 Molecule (CD33) Antikörper

Antigen: CD33 Molecule (CD33)

Synonyme: CD33, p67, SIGLEC3, FLJ00391, SIGLEC-3

Klonalität: Monoklonal (WM53)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Kostimulation

Produktnummer: ABIN157942

Produktbeschreibung

Weitere Bezeichnung: CD33

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: WM53

Beschreibung: CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity. Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells Function: Adhesion and lectin activity Synonyms: Siglec-3, gp67, p67 Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting, induction of cytokine production, and immunohistochemical staining of acetone-fixed frozen tissue sections. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157942). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes, monocytes and granulocytes stained with LEAF(TM) purified WM53, followed by anti-mouse IgGs FITC

Publikationen

Publikationen: Favaloro, Bradstock, Kabral et al.: "Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33." in: British journal of haematology, Vol. 69, Issue 2, pp. 163-71, 1988 (PubMed).

Freeman, Kelm, Barber et al.: "Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules." in: Blood, Vol. 85, Issue 8, pp. 2005-12, 1995 (PubMed).

Garnache-Ottou, Chaperot, Biichle et al.: "Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells." in: Blood, Vol. 105, Issue 3, pp. 1256-64, 2005 (PubMed).