CD29 Antikörper

Antigen: CD29

Klonalität: Monoklonal (TS2-16)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Aktivierung (Act)

Produktnummer: ABIN157896

Produktbeschreibung

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: TS2-16

Beschreibung: CD29 is a 130 kD single chain type I glycoprotein, known as integrin beta1, VLA-beta chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin alpha1-alpha6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins which include CD29 bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions. Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells Function: Cell-cell and cell-matrix interactions Synonyms: Integrin beta1 chain, VLA-beta chain, gpIIa Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (alpha1-alpha6), also associates with CD51 (alphaV), and alpha7- alpha9, 130 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, and activation of integrin beta1. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157896). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes stained with LEAF(TM) purified TS2/16, followed by anti-mouse IgGs FITC

Publikationen

Publikationen: Hynes: "Integrins: versatility, modulation, and signaling in cell adhesion." in: Cell, Vol. 69, Issue 1, pp. 11-25, 1992 (PubMed).

Hemler: "VLA proteins in the integrin family: structures, functions, and their role on leukocytes." in: Annual review of immunology, Vol. 8, pp. 365-400, 1990 (PubMed).

Hemler, Sanchez-Madrid, Flotte et al.: "Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 3011-8, 1984 (PubMed).

Sanchez-Aparicio, Dominguez-Jimenez, Garcia-Pardo: "Activation of the alpha 4 beta 1 integrin through the beta 1 subunit induces recognition of the RGDS sequence in fibronectin." in: The Journal of cell biology, Vol. 126, Issue 1, pp. 271-9, 1994 (PubMed).

Porter, Hogg: "Integrin cross talk: activation of lymphocyte function-associated antigen-1 on human T cells alters alpha4beta1- and alpha5beta1-mediated function." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1437-47, 1997 (PubMed).

Gutierrez-Lopez, Ovalle, Yanez-Mo et al.: "A functionally relevant conformational epitope on the CD9 tetraspanin depends on the association with activated beta1 integrin." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 208-18, 2002 (PubMed).

Murga, Fernandez-Capetillo, Tosato: "Neuropilin-1 regulates attachment in human endothelial cells independently of vascular endothelial growth factor receptor-2." in: Blood, Vol. 105, Issue 5, pp. 1992-9, 2005 (PubMed).

Frank, Margaryan, Huang et al.: "ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma." in: Cancer research, Vol. 65, Issue 10, pp. 4320-33, 2005 (PubMed).

Conway, Petrovic, Li et al.: "Prostate-specific membrane antigen regulates angiogenesis by modulating integrin signal transduction." in: Molecular and cellular biology, Vol. 26, Issue 14, pp. 5310-24, 2006 (PubMed).