CD28 (CD28) Antikörper

Antigen: CD28 (CD28)

Synonyme: Tp44, MGC138290

Klonalität: Monoklonal (CD28-2)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Kostimulation, Immunpräzipitation (IP), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN157880

Produktbeschreibung

Weitere Bezeichnung: CD28

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: CD28-2

Beschreibung: CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subset, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered as a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. Distribution: Mature T cells, thymocytes, NK cell subset, plasma cells, EBV-positive B cells Function: T cell costimulation Synonyms: T44, Tp44 Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation . The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157880). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4°C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes stained with LEAF(TM) purified CD28.2, followed by anti-mouse IgGs FITC

Publikationen

Publikationen: Linskey, Lunsford, Flickinger et al.: "Stereotactic radiosurgery for acoustic tumors." in: Neurosurgery clinics of North America, Vol. 3, Issue 1, pp. 191-205, 1992 (PubMed).

June, Bluestone, Nadler et al.: "The B7 and CD28 receptor families." in: Immunology today, Vol. 15, Issue 7, pp. 321-31, 1994 (PubMed).

Nunes, Klasen, Franco et al.: "Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity." in: The Biochemical journal, Vol. 293 ( Pt 3), pp. 835-42, 1993 (PubMed).

Galea-Lauri, Darling, Gan et al.: "Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 62-70, 1999 (PubMed).

Tazi, Moreau, Bergeron et al.: "Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3511-5, 1999 (PubMed).

Scharschmidt, Wegener, Heissmeyer et al.: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004 (PubMed).

Jeong, Qiao, Nascimbeni et al.: "Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates." in: Journal of virology, Vol. 78, Issue 13, pp. 6995-7003, 2004 (PubMed).

Rivollier, Mazzorana, Tebib et al.: "Immature dendritic cell transdifferentiation into osteoclasts: a novel pathway sustained by the rheumatoid arthritis microenvironment." in: Blood, Vol. 104, Issue 13, pp. 4029-37, 2004 (PubMed).