CD14 Molecule (CD14) Antikörper (Alexa Fluor 700)

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (M5E2)

Wirt: Maus

Reaktivität: Human, Cynomolgus, Hund, Rhesusaffen

Konjugat: Alexa Fluor 700

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN157714

Produktbeschreibung

Weitere Bezeichnung: CD14

Isotyp: IgG2a, kappa chain

Klon: M5E2

Beschreibung: CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Distribution: Monocytes, macrophages, granulocytes (low) Function: LPS receptor, clearance of Gram-negative pathogens Synonyms: LPS receptor Structure: GPI-linked membrane glycoprotein, 53-55 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157702). This reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is < 2.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 700.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-CD14 Molecule (CD14) antibody (Alexa Fluor 700)

Publikationen

Publikationen: Stocks, Albrechtsen, Kerr: "Expression of the CD15 differentiation antigen (3-fucosyl-N-acetyl-lactosamine, LeX) on putative neutrophil adhesion molecules CR3 and NCA-160." in: The Biochemical journal, Vol. 268, Issue 2, pp. 275-80, 1990 (PubMed).

Williams, Corey, Westmoreland et al.: "Perivascular macrophages are the primary cell type productively infected by simian immunodeficiency virus in the brains of macaques: implications for the neuropathogenesis of AIDS." in: The Journal of experimental medicine, Vol. 193, Issue 8, pp. 905-15, 2001 (PubMed).

Power, Wang, Manning et al.: "Bacterial lipoprotein delays apoptosis in human neutrophils through inhibition of caspase-3 activity: regulatory roles for CD14 and TLR-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 8, pp. 5229-37, 2004 (PubMed).

Iwamoto, Iwai, Tsujiyama et al.: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007 (PubMed).