CD14 Molecule (CD14) Antikörper (APC)

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (M5E2)

Wirt: Maus

Reaktivität: Human, Cynomolgus, Hund, Rhesusaffen

Konjugat: APC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN157700

Produktbeschreibung

Weitere Bezeichnung: CD14

Isotyp: IgG2a, kappa chain

Klon: M5E2

Beschreibung: CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Distribution: Monocytes, macrophages, granulocytes (low) Function: LPS receptor, clearance of Gram-negative pathogens Synonyms: LPS receptor Structure: GPI-linked membrane glycoprotein, 53-55 kD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation, and immunofluorescence microscopy. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157702). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinigung: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Lagerung: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood monocytes stained with M5E2 APC

Publikationen

Publikationen: Stocks, Albrechtsen, Kerr: "Expression of the CD15 differentiation antigen (3-fucosyl-N-acetyl-lactosamine, LeX) on putative neutrophil adhesion molecules CR3 and NCA-160." in: The Biochemical journal, Vol. 268, Issue 2, pp. 275-80, 1990 (PubMed).

Williams, Corey, Westmoreland et al.: "Perivascular macrophages are the primary cell type productively infected by simian immunodeficiency virus in the brains of macaques: implications for the neuropathogenesis of AIDS." in: The Journal of experimental medicine, Vol. 193, Issue 8, pp. 905-15, 2001 (PubMed).

Power, Wang, Manning et al.: "Bacterial lipoprotein delays apoptosis in human neutrophils through inhibition of caspase-3 activity: regulatory roles for CD14 and TLR-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 8, pp. 5229-37, 2004 (PubMed).

Iwamoto, Iwai, Tsujiyama et al.: "TNF-alpha drives human CD14+ monocytes to differentiate into CD70+ dendritic cells evoking Th1 and Th17 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1449-57, 2007 (PubMed).