Integrin, alpha M (Complement Component 3 Receptor 3 Subunit) (ITGAM) Antikörper

Antigen: Integrin, alpha M (Complement Component 3 Receptor 3 Subunit) (ITGAM)

Synonyme: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Klonalität: Monoklonal (CBRM1-5)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Blockierende Antikörper (Inhibition)

Produktnummer: ABIN157664

Produktbeschreibung

Weitere Bezeichnung: CD11b (activated)

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: CBRM1-5

Beschreibung: The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as alphaM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin beta2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize non-activated CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the alpha chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1. Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation Synonyms: Integrin alphaM chain, C3biR, CR3, Mac-1, Mo1 Structure: Integrin, type I transmembrane glycoprotein, associates with integrin beta2 (CD18), 165-170 KD

Anwendungen

Applikationshinweise: Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking of the binding of ICAM-1 and fibrinogen. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157664). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at < 1.0 µg per 10 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Diamond, Springer: "A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen." in: The Journal of cell biology, Vol. 120, Issue 2, pp. 545-56, 1993 (PubMed).

Stewart, Thiel, Hogg: "Leukocyte integrins." in: Current opinion in cell biology, Vol. 7, Issue 5, pp. 690-6, 1996 (PubMed).

Petty, Todd: "Integrins as promiscuous signal transduction devices." in: Immunology today, Vol. 17, Issue 5, pp. 209-12, 1997 (PubMed).

Bryn, Mahic, Enserink et al.: "The cyclic AMP-Epac1-Rap1 pathway is dissociated from regulation of effector functions in monocytes but acquires immunoregulatory function in mature macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 12, pp. 7361-70, 2006 (PubMed).

Sithu, English, Olson et al.: "Membrane-type 1-matrix metalloproteinase regulates intracellular adhesion molecule-1 (ICAM-1)-mediated monocyte transmigration." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 25010-9, 2007 (PubMed).