CD3 (CD3E) Antikörper

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (HIT3a)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Aktivierung (Act), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN157486

Produktbeschreibung

Weitere Bezeichnung: CD3

Format: Low endotoxin (sterile), Low-endotoxin, azide-free (LEAF)

Isotyp: IgG2a, kappa chain  (Passende Sekundärantikörper)

Klon: HIT3a

Beschreibung: CD3epsilon is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3epsilon, one CD3gamma, one CD3delta, one CD3zeta (CD247), and a T-cell receptor (alpha/beta or gamma/delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The HIT3a antibody is able to stimulate T cell activation. Distribution: Mature T and NK-T cells, thymocyte differentiation Function: Antigen recognition, signal transduction, T cell activation Synonyms: T3, CD3epsilon Structure: Ig superfamily, with the subunits of CD3gamma, CD3delta, zeta (CD247) and TCR (alpha/beta or gamma/delta) forms CD3/TCR complex, 20 kD

Anwendungen

Applikationshinweise: Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes. The LEAF(TM) purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm sterile-filtered) is recommended for functional assays (ABIN157486). Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is < 0.5 µg per 10 cells in 100 µl volume or 100 µl of whole blood. For activation of T cells with immobilized HIT3a, the suggested use of this reagent is 10 µg/ml for coating. With the soluble format (in the presence of Fc receptor-bearing accessory cells), the suggested use is 0.01 - 0.1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Reinheit: LEAF

Reinigung: The antibody was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Human peripheral blood lymphocytes stained with LEAFTM purified HIT3a and detected with anti-mouse IgGs FITC

Publikationen

Publikationen: Lanier, Allison, Phillips: "Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 8, pp. 2501-7, 1986 (PubMed).

Sedelies, Sayers, Edwards et al.: "Discordant regulation of granzyme H and granzyme B expression in human lymphocytes." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26581-7, 2004 (PubMed).

Scharschmidt, Wegener, Heissmeyer et al.: "Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3860-73, 2004 (PubMed).

Rivollier, Mazzorana, Tebib et al.: "Immature dendritic cell transdifferentiation into osteoclasts: a novel pathway sustained by the rheumatoid arthritis microenvironment." in: Blood, Vol. 104, Issue 13, pp. 4029-37, 2004 (PubMed).

Smeltz: "Profound enhancement of the IL-12/IL-18 pathway of IFN-gamma secretion in human CD8+ memory T cell subsets via IL-15." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 8, pp. 4786-92, 2007 (PubMed).